Study On The Mechanism Of Anti-tumor Action And Quality Control Of Ganoderma Lucidum Spores

AIM: This study was designed to evaluate the mechanism of anti-tumor action of Ganoderma Lucidum Spores on Hepatocarcinoma. In vitro study, the effect of Ganoderma Lucidum Spores on the growth and cell cycle of HepG₂ cells was detected. And in vivo, the nude mice model of the subcutaneous transplanting tumor was established to evaluate the inhibitory effect. Then immunohistochemistry was used to evaluate the antiangiogenesis on the tumors of nude mice. Meanwhile, triterpene constituents and polysaccharides in Ganoderma Lucidum Spores were determined by HPLC and UV-VIS.METHODS: The HepG₂ cells were treated with Ganoderma Lucidum spores in four different concentrations (2500μg/ml, 1250μg/ml, 625μg/ml and 312.5μg/ml) for 48, 72 and 96hours, and then measured by MTT assay to detect the inhibitory rates. The HepG₂ cells were also treated with Ganoderma Lucidum spores in two different concentrations (3mg/ml, 6mg/ml) for 48hours, and examined by flow cytometry to detect the effects on cell cycle. After model of the subcutaneous transplanting tumor was established by HepG₂ cells, the nude mice were randomly divided into five...