Studies On Preparation And Modification Of Oligosaccharides From Ganoderma Lucidum Spores

In this paper, SDGLS (sporoderm-broken and defatted Ganoderma lucidum spores) provided by Kaiping healthwise Co., LTD was taken as the raw material. Carboxy methyl SDGLS was prepared by carboxy methylated reaction. And carboxy methyl oligosaccharides were made by the enzyme degradation of Carboxy methyl SDGLS.1 The raw material was carboxy methylated modified. Taking the DS (degree of substitution) as the standard, the reaction conditions were optimized. The results indicated the optimal conditions: the SDGLS was 1g, the concentration of thanol was 80%, the dose of sodium hydroxide was 1.20g, the dose of chloroactic acid was 1.18g, alkalization was 2h, reaction temperature was 45℃, and etherification time was 20h. In these conditions, the DS of carboxy methyl reached to 0.78. Then we studied the factors which would influence the solubility of the modified samples. And the results indicated the optimum solution conditions: pH5.15, DS 0.78(the maximum value of DS), temperature was 50℃and solution time was 3d. Three kinds of methods were used to extract the saccharides. There were water boiling method, freeze thaw and water boiling method, ultrasonic and water boiling method. And the extraction yields of these methods were: 3.12%, 3.68% and 4.36%.The sample, whose DS was 0.78, was dissolved in these conditions and its extraction yield of total saccharides was 24.6%, which was 7 times more than water boiling method's.2 The total saccharides contents of the sample solution and its liquid supernatant were determined using the phenol-sulfuric method. The results showed that the total saccharides contents were 26.7% and 23.8%. The oligosaccharides with carboxymethyl group were prepared by cellulose enzyme degradation. In order to get the oligosaccharides with a certain range (6～10) of polymerization degree, the conditions of enzyme degradation were studied. The appropriate conditions were that pH was 4.4, temperature was 50℃, concentration of the enzyme was 200U/g of substrate, and time was 2h. In these conditions, the oligosaccharides with an average polymerization degree of 7.7 were abtained.3 The Bifidobacterium was proliferation in vitro taking glucose with the concentration of 1% and homemade oligosaccharides with different concentrations as the carbon source. And we studied its proliferation situation by determining the pH, the absorbance at 620nm and the bacteria concentration of the culture solution. The results showed that the effect of homemade oligosaccharides on proliferation of Bifidobacterium was obvious and the effect would become better and better with the concentration of oligosaccharides increasing. From 0.5% to 3%, its growth times would be 2.2, 2.9, 3.3, 3.6. And when their concentrations were the same, the effect of glucose would be better than homemade oligosaccharides.4 A preliminary industrial design was made for Carboxy methyl SDGLS and carboxy methyl oligosaccharides. The main jobs were: process, key parameters, workshop design. And this design provided a certain basis for their industrial production.