Expression Of P57～(KIP2) And PCNA In Human Gastric Carcinoma And Its Clinical Significances

Objective:The balance of cell proliferation and apoptosis is regulated precisely by cell cycle. Cyclin and Cyclin-dependent kinases positively regulate cell cycle, however Cyclin-dependent kinases inhibitor plays a negative role. The abnormality of cell cycle can lead to the disbalance between cell proliferation and apoptosis. As a result, cell grows out of control and is finally caused to transform into malignancy. P57^KIP2 is a cancer suppressor gene, and the P57^KIP2 protein, a member of the CIP/KIP family, is a Cyclin-dependent kinases inhibitor. P57^KIP2 protein plays an important role in cell cycle progression by inhibiting cell transform from G1 to S phrase. PCNA is a positive cell cycle regulator, which can promote cell to transform from G1 to S phrase, and can be used as a better index of cell proliferation activity. The purpose of our study is to detect the expressions of P57^KIP2 and PCNA in human gastric carcinoma and to evaluate effects of P57^KIP2 protein and PCNA on the genesis and procession of gastric carcinoma.Methods: 57 cases of gastric carcinoma tissues with clear diagnosis and 48 cases of peri-carcinoma tissues were obtained by gastroscope biopsy. Immunohistochemistry was used to examine the expressions of P57^KIP2protein and PCNA in these tissues. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expressions of P57^KIP2 mRNA in 24 cases of gastric carcinoma tissues and 15 cases of their peri-carcinoma tissues. SPSS 11.0 was used to analyze the correlation of the proteins expressions to clinicopathological parameters.