The Protection Of Naloxone Hydrochloride On Kindey Injury Of Brain-dead Rats

Objective:To investigate the protective effects of Naloxone Hydrochloride(NLX) on the kidney injury of brain-dead rats.Materials and methods: Thirty Wistar rats were randomized into 3 groups: brain-dead group (group B), NLX pretreatment group (group N), and control group (group C), 10 rats in each group, control group (group C, n=10) was maintained anesthesia for 12h but not created brain-dead model; brain-dead group (group B, n=10) was established brain-dead model by increasing intracranial pressure in a modified, slow and intermittent way, and maintained brain-dead state for 12h by respiration and circulation support; NLX pretreatment group (group N, n=10), We began to perform NLX of intraperitoneal injection preoperative 30 min. NLX was induced brain-death as group B and maintained for 12h.NLX is presumed an inhibitor of TNF-α . The protection of NLX is activitied through inhibiting the activity of TNF-α, discontinuing cascadely to activitied ICAM-1, IL-1β,IL-6 and so on.Thus protect brain death donor.The criteria for brain death were as following: a) deep coma; b) papillary reflex and corneal reflex disappear; c) spontaneous respirations cease, and P_aO₂ and saturation oxygen are maintained by a respirator; d) EEG is static; e) atropine test is negative; f) after 12h, the conditions that meet the first 5 criteria are reaffirmed. When the mean artery pressure (MAP) ≥ 60mm Hg, saturation oxygen≥95%, P_aO₂≥100mm Hg, body temperature≥35℃, central venous pressure≤ 10¹2mm Hg, and hemodynamic was stable, the brain-dead models were considered suitable as the brain-dead donor. MAP, heart rate, and central venous pressure of 3 groups were monitored; the parameters for judging brain death in group B...