| Objective: To observe how Bushen formula to regulate hematopoiesis regulation factors of chronic aplastic anemia patients,and discuss the regulation mechanism of kidney promote herbs. Methods: 65 patients with chronic aplastic anemia were randomly divided into two groups.The treatment group(35 cases, including 9 cases of kidney-Yang deficiency,14 cases of kidney-Yin deficiency,12 cases of kidney-Yin and Yang deficiency) was treated with Bushen formulaâ… ,â…¡,â…¢combined with Stanozolol and the control group(30 cases) was treated with Stanozolol for 6 months. The clinical effect were observed during the course. Bone marrow cultivated upper clear fluid and bone marrow monocyte of all patients before and after therapy were collected.Besides these 10 cases of healthy bone marrow were collected as normal controls. Hematopoiesis regulation factors such as EPO,SCF,GM-CSF and mRNA semi-quantitative expression were observed by ELISA and RT-PCR. Results:(1) The total effective rate of the treatment group reaches 80%, while the control group reaching 56.67%, which shows significantly difference between them(P<0.05). Kidney promote herbs can enhance WBC,Hb,PLT of chronic aplastic anemia patients,improve bone hematopoiesis and clinical manifestations markedly.(2) The level of EPO,SCF,GM-CSF in chronic aplastic anemia patients was lower obviously than normal controls(P<0.01),which increased evidently after therapy. Kidney-Yang deficient patients precede kidney-Yin on curative effects.Conclusion: The disorder of immunity mechanism on hematopoiesis regulation factors was one of the pathology mechanism with chronic aplastic anemia patients. Kidney promote herbs can regulate the disorders of immunity.The level of GM-CSF,EPO,SCF increased after therapy.Maybe the mechanism of curative effect was concerned with the regulation of hematopoiesis cytokine.
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