Expression And Clinical Significance Of Survivin Gene And CD34 In Acute Leukemia Patients

Objective This study was aimed to investgate the expression, clinicalsignificance and correlation of survivin gene and CD34 in acute leukemia (AL)patients, to direct individual chemotherapy for acute leukemia patients and toprovide bases for survivin gene target treatment of AL patients.Method The expression levels of survivin mRNA in bone marrow mononuclearcells (BMMNC) from 55 AL patients and 15 control cases were detected bysemi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Theexpression levels of CD34 from 55 AL patients were detected by flow cytometry(FCM).Result1. Survivin mRNA positive expression rates of AL, ALL and ANLL patients were significantly higher than that in control group (67.27%, 76.92% and 64.29% vs. 20.00%, P<0.05).2. The semi-quantitative expression levels of survivin mRNA in AL, ALL and ANLL patients were also significantly higher than that in control group(0.61±0.19, 0.65±0.11 and 0.60±0.21 vs. 0.31±0.03, P＜0.05).3. There was no significant difference of survivin mRNA positive expression rates and levels between ALL and ANLL patients (P>0.05).4. There was no significant difference of survivin mRNA positive expression rates between patients accepted initial treatment and relapsed (66.67% vs. 70.00%, P<0.05), but the expression levels of survivin mRNA in the patients relapsed were...