The Study Of Expression And Clinical Significance Of Survivin Gene In Patients With Acute Leukemia

Objective To observe expression of survivin gene in diverse group acute leukemia andacute leukemic different phase, combining with change of peripheral white blood cellscount and cytogenetics, explore the correlation between expression of survivin gene inacute leukemic cells and its occurrence, development, treatment and prognosis.Methods By using semi-quantiative reverse transcriptase polymerase chain reaction(RT-PCR) technique, survivin gene expression in 62 acute leukemia patients(includingpreliminary diagnosis group, release group and relapse group) was analysed.At the sametime, peripheral white blood cells count and chromosome karyotype analysis weredetermined, contrasted with 10 health adult.Results Survivin gene expression positive-rate in cells of acute leukemia patients atdiagnosis was 72.58％. Survivin gene expression positive-rate was 76.47％(13/17) inALL patients and 71.11％(32/45) in AML patients, respectively. The expression positive-rate was higher in ALL and AML cells than that in normal bone marrow cells[20.00％(2/10)], (x~2=6.01, 7.02, P＜0.05). Semi-quantiative results of survivin/β-actingwere 0.68±0.18, 0.73±0.14, 0.30±0.04 in ALL, AML patients and health adult,respectively. The expression level was higher in ALL and AML cells than that in normalbone marrow cells (F=7.80, q=4.70, 5.54, P＜0.05).The expression positive-rate ofprimary cured patients(78.95％)and relapse patients (85.71％)was significantly higherthan that in normal bone marrow cells(x~2=6.70,5.53, P＜0.05). The expression positivepatients in abnormal extent of peripheral white blood cells counts(40/48) weresignificantly higher than that in normal extent of peripheral white blood cells counts(5/14)(x~2=10.07, P＜0.05). The survivin expression positive patients of abnormal chromosomekaryotype analysis (44/54) was significantly higher than that of normal chromosomekaryotype (1/8) (x~2=13.37, P＜0.05).The BMR was higher in patients of survivinmRNA negative expression than that in positive expression patients after one course ofchemo-treatment (87.5％vs 30.0％, x~2=6.37, P＜0.05).Conclusion Higher expression level of survivin mRNA may indicate the correlationbetween expression of survivin gene in acute leukemia and its occurrence anddevelopment, which may play an important role in proliferation and differentiation ofacute leukemic cells. Survivin gene may result in changes of peripheral white blood cells count and cytogenetics and be one of the reasons that leukemic cells are insensitive tochemotherapy.