The Preparation And Characteristic Evaluation Of Bone Matrix/PLA Bone Repair Material By Supercritical CO₂ Fluid Technique

The material with excellent biocompatibility, which is the key to clinical applications, is a popular topic among the bone repair materials.In this research, a novel method was introduced to treat xenogenic bone, which integrates Supercritical Carbon Dioxide Fluid Technology (SC-CO₂-FT) and chemical ultrasound marination. A series of xenogenic bone materials were prepared using this methodology. The treated bone materials were determined by scanning electron microscopy (SEM), X-ray diffraction, FT-IR and thermo gravimetric analyses (TG). The treated bone material could maintain its natural porous structure, and the wall of it was quite clear, the results of FT-IR and TG indicated that the treatments had removed almost all the organic component from the xenogenic bone. Moreover, the structure of the hydroxyapatite crystals was retained well. The in vitro results showed that the new xenogenic matrix maintains the good biocompatibility. The results showed that these methods are promising in treating xenogenic bone.Based on these results, a series of novel allogenic bone matrix/ PLA (poly D,L-lactide), xenogenic bone matrix /PLA, bone decalcified matrix/PLA and bone matrix gelatin/PLA composite scaffolds, were prepared by supercritical carbon dioxide fluid technology ((SC-CO₂-FT). The composites were determined by SEM, porosity analysis, in vitro and in vivo studies. The bone matrix material had improved a great in the aspect of mechanics character and the biocompatibility, the composites had a interconnected porous structure with the porosity over 80%, furthermore, the most outstanding feature of this composite is that a large number of micropores (diameter=10-20μm) were generated on the tri-dimensional pores surface while avoiding the use of organic solvent, the pore formed by SC-CO₂ technique is appropriate to cell ingrowth. The in vitro showed that these composites have good...