The Xenogeneic Bone Graft Material For The Repair Of Rabbit Radius Defection Experimental Study

ObjectTo observe the ability of hydrogen peroxide and pepsin deproteinized xenogeneic bone graft material for repairing bone defection of rabbit radius.Method75New Zealand rabbits were randomly divided into5groups, including blank group, autogenous bone group, bone matrix group, hydrogen peroxide group, pepsin group. Each group had15rabbits. Operation caused rabbit left forelimb segment bone defect of radius10mm.The blank group didn’t implant any material in bone defect. Other groups are respectively in bone defect at the implantation of autogenous bone,bone matrix, hydrogen peroxide or pepsin deproteinized xenogeneic bone.5animals were sacrificed in each group after4weeks,8weeks and12weeks. Took X-ray on operation site. Performed the Micro-CT test and histological observation.ResultsAfter12weeks the X-ray showed the blank group bone defects without callus formation. The bone defect had healed completely in autogenous bone group. The bone matrix group and pepsin group were more obvious in new bone formation than hydrogen peroxide group. The Micro-CT displayed that bone quality of autogenous bone group was obviously better than the other groups.The bone matrix group and pepsin group’s bone volume fraction and trabecular bone number were greater than hydrogen peroxide group. The pepsin group’s bone density and trabecular bone thickness were greater than hydrogen peroxide group. The hydrogen peroxide group’s trabecular bone gap and structure model index were greater than bone matrix group and pepsin group. Histological observation displayed that the marrow cavity had recanalized and the cortical bone had shaped good in autologous bone group, bone matrix group and pepsin group were more obvious in osteogenic activity than hydrogen peroxide group.ConclusionThe osteogenic ability of autogenous bone group was better than the other groups. The osteogenic ability of bone matrix group and pepsin group was better than hydrogen peroxide group. There was not significant difference of osteogenic ability between bone matrix group with pepsin group.