Expression Of VEGF And MVD In Nonneoplastic Epithelial Disorders Of Vulva,VIN And Vulvar Squamous Carcinoma

VSCC is the most common vulvar carcinoma, accounting for about 80%～90% of the vulvar malignant tumor,more common in women over the age of 60,it's incidence has increased in recent years.NNEDV (including SH and LS) and VIN both can be developed into VSCC.VEGF is the most powerful angiogenesis factor, is the only one to directly stimulate endothelial cells proliferation of endothelial cell-specific mitogen. It's relative molecular mass is 34 000~45 000 ,and it's genes on chromosome 6 at the P21.3. VEGF can expression in lots of normal adult and animal tissues, but the expression level is very low. In the condition of pathology, especially in malignant tumour, VEGF expression is often excessive, whose expression levels and microvessel density are positively correlated with tumor's malignant, show through its promotion of angiogenesis can promote tumor's growth.CD34 is a relative molecular mass of 105~120kD Glycosylation transmembrane glycoprotein, with adhesion, accelerated vascular endothelial cells gathered before the formation of blood vessels and control the proliferation of hematopoietic cell differentiation and function opt to the human and mouse hematopoietic stem / progenitor cells expression also in the vascular endothelial cells and other tissues, CD34 antigen endothelial cells in the capillary, small vascular endothelial cells were strongly positive stability, which is concerned the most reliable marker of the vascular endothelial cells recently.We chose two angiogenesis factor VEGF and CD34 to study the angiogenesis's significance in the process of vulvar canceration. At present,domestic and foreign research even less, but in general ,VEGF expressed in VSCC was high,in VIN was low; However, there were divarications exist in the expression of LS, Farrell AM think that the VEGF expressed in LS had no difference with the NS,but Yang Jing held that the VEGF expressed in LS was higher than NS, and LS had the compensatory promotion angiogenesis factors.OBJECTIVE: Examining the expression of VEGF and MVD in NNEDV,VIN and VSCC ;To investigate the expressive rules of VEGF and MVD ,and the significance in vulvar cancerization.METHOD: Immunohistochemical demonstration(S-P) of VEGF and MVD in 20 cases of LS ,20 cases of SH,20 cases of VIN,20 cases of VSCC and 10 cases of NS(form the patients of vulvar repair)。Use the SPSS11.5 to analysis.RESULT:(1) The expression of VEGF was not found in NS, LS and SH;In VIN,VEGF expressed in vulvar dermal scattered cells within the cytoplasm was brown granules, The positive rate was 35% (7/20), which was significantly higher than that of NS,LS and SH, the difference was statistically significant (P<0.05);In VSCC the pathways expressed in the cytoplasm of tumor cells was brown and concentrated ,positive rate was 80% (16/20) significantly higher than NS, SH, LS and VIN, the difference was statistically significant (P <0.05). (2) CD34 was localized in the cytoplasm of endothelial cells or membrane. Freshmen in the microvascular and non-angiogenesis including vascular endothelial cells were positive expression was light brown or dark brown granules.May better reflect the MVD in vulvar dermis. In NS,MVD value was 20.80;InSH,MVD value was 29.45;In LS,MVD value was 17.40;In VIN,MVD value was 38.90;In VSCC,MVD value was 46.85.The vulvar lesions and NS were significantly different(P <0.05),and there was a significant difference between every two groups.(P <0.05).CONCLUSION: (1)The results of this study showed that there was no VEGF expression in NS, LS and SH,Indicate that angiogenesis in NS,LS and SH was low; VEGF in VIN was slightly expression, in VSCC showed strong expression further illustrate the VEGF promoted the angiogenesis of VIN and VSCC, And made an important role in vulvar cancerization. (2) MVD in LS is lower than NS,Showed that there was microcirculative handicap in LS;MVD in SH was noticeably more than NS,Indicate that SH had Microcirculation abundant than normal, which maight promote the occurrence of SH;MVD in VIN is higher than NS and NNEDV ,But lower than VSCC,Showed that compare with benign proliferation (such as SH) dermis VIN epithelial abnormal proliferation had more abundant blood supply, blood flow increased, abnormal cells could accelerate growth, or even cancer ; VSCC had the highest MVD, Note that micro-circulation in VSCC was abundant and promote tumor growth.(3)Our result showed that the group of high VEGF,also had high MVD value too, further confirmed that VEGF had close relationship with angiogenesis.