The Expressions And Significances Of RASSF1A And CyclinD1 In Nonneoplastic Epithelial Disorders Of Vulva

Objective: Apoptosis index(AI) of epithelial cells of the vulva was examined by Td T-mediated d UTP Nick-End Labeling(Tunel), and the expression of RAS association domain family 1A(RASSF1A) protein and cell cycle protein D1(Cyclin D1) in nonneoplastic epithelial disorders of vulva(NNEDV) was detected by immunohistochemistry in order to explore the occurrence and development of NNEDV and lay the foundation for pathogenesis and treatment of NNEDV. Methods: 39 cases of vulvar lesions tissues and vulvar skin adjacent to the lesions were collected from NNEDV patients in the Affiliated Hospital of Luzhou Medical College from March 2013 to October 2014. According to clinic pathologic types, these were divided into 24 cases of vulvar squamous hyperplasia(VSH), 10 cases of lichen sclerosus of vulva(VLS) and 5 cases of mixed lesions. AI(positive cells number/total number of cells×100%)of epithelial cells of the lesions group and adjacent to the lesions group were examined by Tunel. The expression of RASSF1 A protein and Cyclin D1 protein in two groups were detected by immunohistochemistry. The mean optical density(MOD) of positive cells in two groups were analyzed by Image Pro6.0 image analysis system. The expression and correlation of RASSF1 A protein and Cyclin D1 in each group were analyzed.The statistical methods were calculated using the SPSS17.0software,and P<0.05 was considered statistically significant.Results:1. AI in the lesions group was higher than the adjacent to the lesions group,and the difference was statistically significant(P<0.05);2.the expression of RASSF1 A protein in the VSH lesions group was lower than that in adjacence to the VSH lesions group, and the difference was statistically significant(P<0.05). The expression of RASSF1 A protein in the VLS lesions group was lower than that in adjacence to the VLS lesions group, and the difference was statistically significant(P<0.05). The expression of RASSF1 A protein in mixed lesions group was lower than that in the adjacent to the mixed lesions group, and the difference was no statistically significant(P>0.05).The difference of RASSF1 A expression in different clinic pathologic types’ s lesions group(VSH,VLS,and mixed lesions) were statistically insignificant(P>0.05). 3.The expression of cyclin D1 protein in the VSH lesions group is higher than that in adjacent to the VSH lesions group, and the difference was statistically significant(P<0.05). The expression of cyclin D1 protein in the VLS lesions group is slightly higher than that in adjacent to the VLS lesions group, and the difference was no statistically significant(P>0.05). The expression of cyclin D1 protein in mixed lesions group was higher than that in the adjacent to the mixed lesions group, and the difference was statistically significant(P<0.05).The difference of cyclin D1 expression in different clinic pathologic types’ s lesions group(VSH,VLS,and mixedlesions) were no statistically significant(P>0.05). 4.AI and expression of RASSF1 A were significantly positively correlated in the VSH lesions group(r=0.628, P=0.001<0.05), VLS lesions group(r=0.928,P=0.00<0.05),mixed lesions group(r=0.935,P=0.02<0.05),and the adjacent to the lesions group(r=0.363, P=0.023<0.05). 5.AI and expression of RASSF1 A were significantly negatively correlated in the VSH lesions group(r=0.628,P=0.001<0.05)and in the adjacent to the lesions group(r=0.363,P=0.023<0.05). They were uncorrelated in the VLS lesions group(r=0.928,P=0.00<0.05) and in the mixed lesions group(r=0.935,P=0.02<0.05). 6.The expressions of RASSF1 A and cyclin D1 protein in the VSH lesions group were significantly negatively correlated(r=-0.711,P<0.01<0.05).Their expressions in the VLS lesions group were uncorrelated(r=-0.464, P=0.177>0.05),the mixed lesions group(r=-0.236, P>0.05) and the adjacent to the lesions group(r=-0.046, P>0.10>0.05).Conclusion: 1.AI in the lesions group was higher than AI in adjacent to the lesions group,and the difference was statistically significant(P<0.05),and AI in different clinic pathologic types’ s lesions groups(VSH,VLS,and mixed lesions) were lower than the corresponding adjacence to the lesions group which was suggested that pathogenesis of NNEDV was closely related to apoptosis.2.The expressions of RASSF1 A protein in the VSH and VLS lesions group are lower than that in corresponding adjacent to the lesions group. The absence of expression ofRASSF1A might block apoptosis pathway of vulvar epithelial cell, abnormal proliferation of vulvar epithelial cell might cases the occurrence of NNEDV lesions.3.The expressions of cyclin D1 protein in the VSH and mixed lesions group were higher than that in corresponding adjacent to the lesions group. Over-expression of cyclin D1 protein induce cell proliferation might participate in the pathogenic process occurrence of VSH and mixed lesions. 4.The expressions of RASSF1 A and cyclin D1 protein in the VSH lesions group were significantly negatively correlated.the absence of expression of RASSF1 A in the epithelial cells of the vulva might increase cyclin D1 protein accumulation, cause decreased apoptosis which might case the occurrence of VSH lesions.The expressions of RASSF1 A and cyclin D1 in VLS lesions and mixed lesions group were uncorrelated,which was suggested that the pathogenesis of VLS and mixed lesions was a process of multifactor,and the specific mechanism remains to be further studied.