Study Of Relation Between Sperm Acrosomal Integrity And Smoking, Drinking And Freeze

Currently, male infertility has been a world matter. In clinical practice, infertility affects approximately 15 % of couples trying to conceive and a male cause is believed to be a sole or contributing factor in approximately half of these cases. Many factors are responsible for male infertility, including inner factor and exoteric factors.The analysis of semen parameters included sperm count, the percentage of normal morphology sperm. But it's not enough for evaluation of male infertility. Enzymes are important for fecundation of sperm and egg. Therefore, if acrosomal integrity were lower, it would influence the binding of sperm and egg.Now, many reports paid attention to the relation between acrosomal integrity and ability of impregnation. There was no clarity for influencing factors of acrosomal integrity, its mechanism, idiographic area, and so on.In the current study, several comparative studies were carried out to clarify whether inner and outer factors influence on acrosomal integrity or not. The sperm morphology analysis using semiautomated sperm morphology analyzer (ASMA) was performed. Patients voluntarily filled out a questionnaire in order to analyze exoteric factors, cigarette and alcohol consumption. Results:①Acrosomal integrity of abnormal morphology sperm group was significantly lower than that of normal morphology sperm group (P<0.05).②Acrosomal integrity of abnormal semen pH group was significantly lower than that of normal semen pH group (P<0.05).③Acrosomal integrity of abnormal semen volume group was no statistics difference in comparison with normal semen volume group (P>0.05).④Acrosomal integrity of abnormal liquefaction time of semen group was no statistics difference in comparison with normal liquefaction time of semen group (P>0.05).⑤Acrosomal integrity of smoking group was significantly lower than that of fertile group (P<0.01). Acrosomal integrity of smoking group was significantly lower than that of nonsmoking group (P<0.05).⑥Acrosomal integrity of drinking group was significantly lower than that of fertile group (P<0.05). Acrosomal integrity of drinking group was no statistics difference in comparison with non-drinking group (P>0.05).⑦After selecting, acrosomal integrity was significantly higher than that of before selecting (P<0.001).⑧After freezing and thawing, acrosomal integrity of rapid and slow freezing methods were no statistics difference in comparison with before freezing (P>0.05). Acrosomal integrity of rapid freezing method was no statistics difference in comparison with slow freezing method (P>0.05).All results showed that some factors influence acrosomal integrity. Acrosomal integrity was consistent with the percentage of normal morphology sperm. Semen pH could make acrosomal integrity decreased. Semen volume and liquefaction time didn't influence acrosomal integrity. Acrosomal integrity is relevant to smoking, drinking. Semen selection of swimming could make acrosomal integrity increased. When glycerol was cryoprotectant, rapid and slow freezing didn't influence acrosomal integrity.