BCSG1,c-erbB-2 And VEGF Expression In Breast Cancer: Correlation With Clinicopathologic Parameters

Objective:Breast cancer specific gene 1(BCSG1) was identified and cloned by direct differential cDNA sequencing in 1997,and defined as a new member of the neural protein synuclein(SNCs) family. BCSG1 has been found express highly in invasive breast carcinoma, while nearly no positive expression in normal breast and benign lesions. Thus, it was named as breast cancer specific gene 1(BCSG1).Recently, it has been reported home and abroad that BCSG1 is a prognostic factor which express partly in ductal carcinomas in situ, but express highly in extensive infiltrative breast cancer ofâ…¢orâ…£stage indicating different proceedings of breast cancer. Researches about c-erbB-2 suggest that it is a high risk factor in carcinogenesis and development of breast cancer,and it can hint poor prognoisis. (vascular endothelial growth factor,VEGF) can stimulate the proliferation and immigration of vascular endothelial cells, promote angiogenesis, increase permeability of blood vessel and strengthen the ability of proliferation and invasion of breast cancer.BCSG1,c-erbB-2,VEGF influence biological behavior respectively in the carcinogenesis and development of breast cancer, inducing malignant transformation, increasing invasiveness, promoting metastasis, accelerating long-term recurrence and degrading long-term survival rate. Up to now, there have not been any research about the correlation of these three proteins and the relationship between the co-expression of these proteins and the clinicopathologic parameters in breast cancer. In this study, we investigated the expression of BCSG1,c-erbB-2,VEGF in human breast cancer tissue by immunohistochemical staining, analyzed their relationship with the clinical pathological features, and evaluated there role in invasion and metastasis of breast cancer, so to provide a basis for predicting breast cancer patients'prognosis.Methods:SP immunohistochemical staining was used to examine the expression of BCSG1,c-erbB-2 and VEGF in 316 breast cancer specimens, randomly selected from January 2004 to December 2004 in Heibei Medical University. The patients were all female, aged 27 to 78(mean 50) years. 187 specimens were infitrating ductal carcinoma, 100 specimens infiltrating lobular carcinoma, and 29 specimens other types(prickle cell carcinoma,secrete lipid carcinoma,mixed pattern carcinoma,mucoepidemoid carcinoma,etc). Histological grade were divided to three grades(except the cases that can not be graded in special category cancer):52 specimens were in grade I, 215 in grade II, and 28 in grade III. Four clinical stages were determined by TNM:45 specimens in stage I,196 in stages II,72 in stages III, and 3 in stagesâ…£. All patients didn't have chemotherapy or radiotherapy before operation. Data collected was analyzed by SPSS12.0.A statistically significant difference was indicated by P<0.05.Enumeration date was analyzed by chi spuare test and ranked data was analyzed by spearman rank correlation.Results:1 The positive expression of BCSG1 was 67.1% in all, with 35.2% showing 1+, 23.7% 2+ and 8.2% 3+. Negative expression accounted 32.9%. There was a positive correlation between the expression of BCSG1 and histological grade,lymph node metastasis and clinical stage (P<0.05),but there wasn't any significant correlation between the expression of BCSG1 and ER,PR,tumor size,menstruation condition and histological category (P>0.05).2 The positive expression of c-erbB-2 was 32.2% in all,with 13.6% showing 1+, 9.8% 2+ and 8.8% 3+.Negative expression accounted 67.8%.There was a positive correlation between the expression of c-erbB-2 and tumor size,histological grade,lymph node metastasis and clinical stage(P<0.05),and there was a negative correlation between the expression of c-erbB-2 and ER,PR(P<0.05). But there wasn't any significant correlation between the expression of c-erbB-2 and menstruation condition and histological category(P>0.05).3 The positive expression of VEGF was 61.0% in all, with 37.0% showing 1+, 18.7% 2+ and 5.3% 3+. Negative expression accounted 39.0%. There was a positive correlation between the expression of VEGF and histological grade,lymph node metastasis and clinical stage (P<0.05),but there wasn't any significant correlation between the expression of VEGF and ER,PR,tumor size,menstruation condition and histological category (P>0.05).4 BCSG1,c-erbB-2 and VEGF expression were positively correlated in breast cancer(P<0.05).5 There was a positive correlation between the co- expression of these proteins and histological grade,lymph node metastasis,clinical stage(P<0.05),but there wasn't any significant correlation between the co-expression and ER,PR,tumor size,menstruation condition,histological category (P>0.05).Conclusions:1 BCSG1,c-erbB-2 and VEGF were all highly expressed in breast cancer. Moreover, the expression sensitivity of BCSG1 was higher than c-erbB-2 and VEGF.2 There was a positive correlation between the co- expression of BCSG1,c-erbB-2,VEGF and histological grade,lymph node metastasis,clinical stage; in addition, the c-erbB-2 expression was positively correlated with tumor sizes, negatively correlated with ER,PR, but there wasn't significant correlation between c-erbB-2 expression and menstruation condition as well as histological category. BCSG1,VEGF expression wasn't correlated with ER,PR,tumor size,menstruation condition and histological category. 3 Strong co-expression of BCSG1,c-erbB-2,VEGF indicated higher malignance,invasion and metastasis.