Study On The Pharmacological Effect And Mechanism Of Anti-hypoxia Components Of Cynomorium Songaricum Rupr.

Objective:To screen the anti-hypoxia components of Cynomorium songaricum Rupr., study the anti-hypoxia pharmacological effect and explore its mechanism.Methods:The anti-hypoxia components of Cynomorium songaricum Rupr. were extracted and separated by position extract and macroporous resin-alcohol gradient elution, and then screened by the anoxic model under the condition of ordinary and low pressure in mice.The anti-hypoxia mechanism was investigated by the anoxic model under the low pressure conditions similar to the plateau enviroment, and systematically evaluated by determining the content of water and lactic acid in cerebral tissue, SOD activity, malondialdehyde (MDA) and protein content in both cerebral tissue and cardiac muscle tissue, as well as plasma glucose level, lipids, levels of plasmatic ACTH and corticosterone, and immune system.According to the purpose of the experiment, the animals were divided into four groups: control group, HYM group, drug treated group (300mg·kg^-1·d^-1×7) and positive control group (TSPG, 300mg·kg^-1·d^-1×7) .All drugs were administered by intragastric administration (i.g.).Results:The survival time of mice under ordinary pressure is longer in three Cynomoriumsongaricum Rupr. treated groups than that of blank control group (P<0.05). Part III(300mg·kg^-1) from water soluble extract of Cynomorium songaricum Rupr. contains thepolyphenol and saponin, has a similar effect of increasing the survival time of mice. After theadministration of Part III, SOD activity in cerebral and cardiac muscle tissue was increased and MDA content was decreased(P<0.05) exposed to hypoxia. Meanwhile, plasma glucose level was increased, the content of water and lactic acid in cerebral tissue was decreased(P <0.05). The pathological study proved that there were some damages in cerebral and cardiac muscle tissue, which had been protected by treatment of PartⅢin hypoxia mice.PartⅢ(300mg·kg^-1·d^-1, i.g.) increased immune organ index, leukocyte and lymphocyte numbers of immunosuppressed mice induced by cyclophosphamide, improved the phagocytosis function of macrophage and serum hemolysin levels. On the other hand, The T-cell proliferation induced by Con A(5μg·mL^-1) was enhanced by serum (5%) in hypoxia mice of Part III treated group, and T-cell proliferation of PartⅢtreated group incubated with serum (5%) in hypoxia mice and Con A(5ug·mL^-1) was enhanced. In addition, the plasma adrenocorticotrophic hormone and corticosterone level in PartⅢ(300mg·kg^-1·d^-1) treated group were decreased compared with hypoxia model group.Conclusion:1 .Cynomorium songaricum Rupr., which is mainly consists of polyphenol and saponin, posseses the anti-hypoxia effect.2.PartⅢcan improve the metabolism of free radical in cerebral and cardiac muscle tissue in hypoxia mice, and can increase the fasting blood glucose level to supply the energy to the critical organ, to protect the damage of cardiac myocytes and brain cells of hypoxia.3. PartⅢhas an improvement of cellular immunity of hypoxia mice and humoral immunity powerful of immunosuppressed mice, and show some protective activation of HPA aixs in hypopxia mice. It was partially certified that the effect of PartⅢon hypoxia mice was carried out by adjusting HPA axis and immune system function to against the damage of hypoxia.