Study On Antioxidant Capacity Of Cynomorium Songaricum Rupr.

Cynomorium Songaricum Rupr. was a special plant in western China. The objective of the research was to trace and screen its antioxidation fraction using both in vivo and in vitro methods, providing some scientific informations on the use of the Cynomorium Songaricum Rupr. resource. The main research contents were as follows:1. Cynomorium Songaricum Rupr. was vibrationally extracted using different solvents including aqueous, aqueous (pH3),60% ethanol,95% ethanol, n-butanol, n-butanol saturated by aqueous and ethyl acetate at room temperature, respectively. And then the different extracts were obtained. The radical scavenging capacities (RSC) of the different extracts were determined spectrophotometrically by UV-DPPH assay. The results indicated that, through synthetic evaluation indexs, all the extracts from Cynomorium Songaricum Rupr. had radical scavenging capacities (RSC), whereas the 60% ethanol extract was the most appropriate extract. Then, the kinetic behavior and main radical scavengers were revealed preliminarily, too.2. Through systematical optimization of the chromatographic condition, a novel HPLC-DPPH method was successfully developed for evaluation of the free radical scavenging activity. The proposed method had good linear dependence, correlation coefficient, RSD(%) of the precision and stability satisfied with analytical method requirement by technology evaluation. Furthermore, the free radical scavenging activities of different 60% ethanol extracts of Cynomorium Songaricum Rupr. were evaluated by the established HPLC-DPPH method. The developed method had important reference values for evaluation and detection of radical scavengers from natural products.3. In order to investigate the most appropriate antioxidation fraction, a futher investigation was carried out. The T-AOC of the extracts were determined to screen the most appropriate extract.40 rats were randomly divided into four groups and then were fed by the most appropriate extract for 4 weeks. The serum was obtained by eye sockets, the activities of MDA, CAT in brain tissue and T-AOC, the DPPH radical scavenging activity in serum were then determined. The results indicated that the activities of MDA (4423.5 g/kg group) in brain tissue were significantly higher than these in control group (P<0.05). Compared with control group, the activities of CAT in the three groups were not increased significantly (P>0.05); Activities of T-AOC (4423.5 g/kg and 8847 g/kg groups) in serum were lower than these in the control group(P<0.05); and the DPPH radical scavenging activities (the 2211.75 g/kg group) increased significantly (P<0.05).4. The radical scavenging capacities (RSC) of Gymnadenia conopsea R.Br. and phallerocarpus gracilis were preliminarily investigated using the UV-DPPH assay, which indicated that they had radical scavenging capacities (RSC).