Expression Of PTTG, BFGF And Endostatin In Colorectal Cancer

Objective: PTTG is a new proto-oncogene with high expression in most malignant tumors. Ever since being discovered, this gene was paid close attention to, and becoming investigation hot spot gradually, especially in cell transformation, blood vessel growth, tumor formation, infiltration and metastasis. bFGF is one of the most important angiogenesis promoters ever known. Endostatin is collagenⅩⅧend fragment. Its molecular mass is 2000. And it is a new factor of antiangiogenesis in clinical test. Oncogenes play the role of tumor angiogenesis in the process of tumor growth. This role is through up-regulating angiogenesis positive factors and / or down-regulating angiogenesis negative factors to achieve. Tumor angiogenes is closely related to its invasiveness, and angiogenesis depends on the balance between endogenous stimulating factors and inhibitors. Immunohistochemical method was used to detect the expressions of PTTG, bFGF and Endostatin and their relationships in colorectal cancer. We approached the effect of PTTG, bFGF and Endostatin in the occurrence of colorectal cancer invasion.Material and Methods: The specimens were taken from Pathology Division of the First Affiliated Hospital of Zhengzhou University from October 2003 to December 2004. They were 57 surgical resected filing wax blocks of colorectal adenocarcinoma, including 28 males and 29 females; The median age was 56 years (31-78 years). By Dukes stage for the A + B 31 cases, C + D 26 cases. 21 cases were well-differentiated, and 36 cases were poorly differentiated. There were 26 cases with lymph nodes metastasis and 31 cases without lymph nodes metastases through conventional lymph nodes check. We used immunohistochemical method to detect the expression of PTTG, bFGF and Endostatin proteins in 57 sections from colorectal cancers, and analyzed the relationship between the protein expression and the clinicopathologic features. Results: 1. The positive rate of PTTG (80.7%) in the period of colorectal adenocarcinoma DukesC+D was higher than the positive rate of PTTG (45.2%)in Dukes A+B.And the difference the difference was statistically significant (P <0.05). The positive rate of PTTG (84.6%) was higher in colorectal cancers with lymph nodes metastasis than the positive rate of PTTG (41.9%) in colorectal cancers without lymph nodes metastasis, and the difference was statistically significant (P <0.05). The expression of PTTG was high in mediate or poorly differentiated colon adenocarcinoma with positive rate 75.0%. The expression of PTTG has nothing to do with gender.2. The positive rate of bFGF (84.6%) in the period of colorectal adenocarcinoma DukesC+D was higher than the positive rate of PTTG (54.8%)in Dukes A+B.And the difference had significance (P<0.05). The expression of bFGF (80.6%) was higher in mediate or poorly differentiated colon adenocarcinoma was higher than in well-differentiated adenocarcinoma tissues, the difference was statistically significant (P <0.05). The positive rate of bFGF (84.6%) was higher in colorectal cancers with lymph nodes metastasis than the positive rate of bFGF (54.8%) in colorectal cancers without lymph nodes metastasis, and the difference was statistically significant (P <0.05). The positive rate of bFGF expression has nothing to do with gender.3. The positive rate of Endostatin (57.7%) in the period of colorectal adenocarcinoma DukesC+D was lower than the positive rate of Endostatin (87.1%) in Dukes A+B.And the difference the difference was statistically significant (P <0.05). The positive rate of Endostatin (53.8%) was lower in colorectal cancers with lymph nodes metastasis than the positive rate of Endostatin (90.3%) in colorectal cancers without lymph nodes metastasis, and the difference was statistically significant (P <0.05). The expression of Endostatin (63.95%) was lower in mediate or poorly differentiated colon adenocarcinoma than in well-differentiated adenocarcinoma tissues, the difference was statistically significant (P <0.05).4. PTTG and bFGF had high expression in colorectal cancer of DukesC+D,but endostatin had low expression in colorectal cancer of DukesC+D. PTTG and bFGF had high expression in colorectal cancer with absorbent gland metastasis, but endostatin had low expression in colorectal cancer with absorbent gland metastasis. PTTG and bFGF had low expression in colorectal cancer without absorbent gland metastasis, but endostatin had high expression in colorectal cancer without absorbent gland metastasis. The expression of PTTG had significant positive correlation with bFGF(γs= 0.702, P<0.05),but the expression of endostatin had significant negative correlation with PTTG and bFGF(γs=-0.392, P<0.05,γs=-0.406, P<0.05).Conclusion: (1) The expression of PTTG is related with Dukes staging , differentiation degree and lymph node metastasis of colorectal cancer , which suggests that PTTG can promote tumor growth, invasion and metastasis. It can be used as a prognostic indicator. (2) PTTG is positively correlated with bFGF and negatively correlated with Endostatin ,which suggests that PTTG can promote colorectal cancer angiogenesis , invasion and metastasis through upregulating the expression of bFGF and / or inhibit the expression of Endostatin .(3) PTTG can be a tumor marker for colorectal cancer detection and a molecule target for therapy.