Expression Of PTTG And FGF-2 In Human Hepatocellular Carcinoma: Relationships To Clinical Tumor Behaviour

Objective: To study the expression of PTTG and bFGF-2 in human hepatocellular carcinoma and their signifecance.Method: PTTG and bFGF-2 were detected by S-P immunohistochemical method in 41 cases of hepatocellular carcinoma and paracancerous tissues, cases of normal liver ;reverse-transcription polymerase chain reaction(RT-PCR) was used to analyzed the level of PTTGmRNA and bFGFmRNA in 3 leases of hepatocellular carcinoma and paracancerous tissues.Results: (1) The positive rate of PTTG protein and bFGF protein in 41 cases of hepatocellular carcinoma and paracancerous tissues were 70.7%(29/41), 90.2%(37/41), 73.1%(30/41), 17%(7/41); In normal tissues the positive rate of PTTG protein were 7.3%(3/41)while bFGF protein was negtive.The difference between them was prominent(p<0.05). 2)Statistical correlation existed between bFGF protein and histological ifferentiation, AFP(+), capsule, tumor thrombi while not with the age and gender; There is no statistical correlation between PTTG and histological differentiation, but it does with capsule, tumor thrombi, AFP(+), lymph node metastasis, hepatic cirrhosis.(3) Compared with normal liver, PTTGmRNA and bFGFmRNA were over expressed in hepatocellularcarcinoma(p<0.05). (4)Correlated studies suggested that ,a correlation between PTTGmRNA and bFGFmRNA. The coefficient of correlation was 0.91.Conclusions: (l)As a novel gene,the over expressions of PTTG may be involed in the progression of HCC and may be asscociated with the early stage of the carcinogenesis.(2)bFGF is over expressed in HCC and can be a potenical marker of HCC.(3)HCC that expressed high levels of PTTGmRNA also exhibited high levels of expression of bFGF,suggesting a correlation between PTTG and bFGF expression,and further suggesting that the PTTG may be involved in tumor angiogenesis and mitogensis.