Effects Of Arsenious Acid On Expression Of PTTG And C-myc In NB4 Cell Line

Objective Observes the arsenic trioxide (As₂O₃) to affect the NB4 cell pituitary tumor transforming gene (PTTG) and the C-myc gene expression influence, is As₂O₃ treats APL to providethe new experimental basis. Methods From Suzhou University purchaser acute promyelocytic leukemia NB4 cell line, carefully cultivating, All test cells were used to grow a few cells. Separately uses the different density As₂O₃ to affect the NB4 cell for24 hours, 48 hours, According to the As₂O₃ intervention time and the density differentgrouping, simultaneously sets up does not add As₂O₃ the interventionthe NB4 cell group, Selects the SMMC-7721 liver cancer cell line to take the masculinecomparison group, each group supposes three duplicate holes, Collects each group of other cells to withdraw total RNA after theTRIzol law to carry on RT-PCR immediately, The PCR product the electricity swims on 1% agrose gelatin, Results Has not added As₂O₃ the NB4 cell comparison group and SMMC-7721 livercancer cell masculine gender comparison group PTTG and the C-myc geneexpression intensity comparison difference does not have Significant (P> 0.05); after 0.5,1.0,2.0μmo1/L As₂O₃ affects NB4 cell 24h, 48h the PTTG gene expression intensity gradually weakens, with has not added As₂O₃ the NB4 cell comparison group comparison difference to have Significant (P <0.05)。The C-myc gene expression intensity also gradually is weaken, with hasnot added As₂O₃ the intervention the NB4 comparison group to compare . P <0.05。Conclusion PTTG and C-myc also have the high expression in the NB4 cell, prompts PTTG and the C-myc high expression arises in the process in APL possibly to play the vital role, As₂O₃ decline NB4 cell PTTG and the C-myc expression function possiblyis it treats one of APL member mechanisms,Its concrete mechanism also needs further to study.