Research Of The Expression Of PTEN In Squamous Cell Carcinoma Of Larynx By The Tissuechip Technology

ObjectiveTo evaluate the expression of PTEN in squamous cell carcinoma of larynx and its relationship with factor like pathologic fractionation,clinical TNM stage,and prognosis by the tissuechip technology.MethodWe studied the expression of PTEN gene and its mRNA on a series of 146 primary laryngeal carcinoma patients, 40 cases of precancerous lesion and 26 cases of vocal fold polyp ,who were undergoing surgical therapy in our hospital from 1990 to 2001, tissuechip by immunohistochemistry and in situ hybridization method. The data observed and some relevant clinical dada were statistically analyzed.Results1. The expression of PTEN in vocal fold polyp was negative.2. The positive expression of PTEN in precancerous lesion and laryngeal carcinoma were 40 % and 43.15 % respectively by immunohistochemistry, and were 72.5%and 59.59% respetively in situ hybridization.3. The difference between the expression of PTEN in laryngeal carcinoma and its pathological fraction and prognosis was statistically significant,but was not significant between that and location, clinical stage and LN metastasis.4. The mRNA expression of PTEN was higher than that of the protein expression in precancerous lesion and cancer tissue.Conclusion1. The tissue microarray technique required shorter time and less expense, and showed higher consistency in our essays.2. And the present study suggests PTEN was a prognosis factor of the Laryngeal carcinoma.