| ObjectiveTo investigate the expression of CD45RO~+ T cell and CD68~+ cell in the lesion of non-neoplastic epithelial disorders and cancer of the vulva.MethodsThe expression of CD45RO~+ T cell and CD68~+ cell were detected with S-P immunohistochemical method in the lesions of 20 non-neoplastic epithelial disorders (including vulvar squamous cell hyperplasia(SH) and vulvar lichen sclerosus(LS)) and vulvar cancer, respectively, and 10 cases of healthy vulvar skin were selected as control.ResultsThe expression of CD45RO~+ T cell and CD68~+ cell in healthy vulvar skin was very low and significantly lower than that in SH, LS and vulvar cancer (P < 0.05). The expression of them in later stage of LS was significantly higher than that in early stage (P < 0.05), and so did in well/moderately differentiated vulvar cancer than that in poorly differentiated ones (P < 0.05). The expression of CD45RO~+ T cell was highest in well/moderately differentiated vulvar cancer, and then in later stage of LS (P < 0.05). However, the expression of CD68~+ cell was highest in well/moderately differentiated vulvar cancer and later stage of LS, but there was no significant difference between them (P > 0.05). The expression of CD68~+ cell in both well/moderately differentiated vulvar cancer and later stage of LS were significantly higher than that in SH, poorly differentiated vulvar cancer and early stage of LS (P < 0.05), but there was no significant difference between SH, poorly vulvar cancer and early stage of LS (P > 0.05). The expression of CD68~+ cell has a positive correlation with CD45RO~+ T cell in the different lesions of vulva, the correlation coefficient was 0.742 (P < 0.05). The CD68~+ cell often appeared around the CD45RO~+ T cell in the lesions and most occurred at the superficial layer.ConclusionCD45RO~+ T cell and CD68~+ cell take part in the immune reaction in the skin, and act as immunological regulation during development of non-neoplastic epithelial disorders and cancer of the vulva. |
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