| Object To research the inhibiting effect of acteoside extracted chemical composition from cistanche tubulosa on begnin prostatic hyperplasia(BPH).Methods The BPH rat model were induced by testosterone propionate,and three dose groups of acteoside (60,30,15mg?kg-1?d-1) were administrated for six weeks,prepared paraffin sections and observed the morphological change of the prostate gland by the microscope. Exzamined AR and TGFβR1 expressions by the immunity histochemistry.Determinated the rate of prostate apoptosis by FCM,with transmission electron microscopy observed the prostate cells ultrastructure,and count the number of glands under the same magnification.Results①The middle and high dose acteoside could obviously reduce the prostate gland fresh weight index ( 0.589±0.001,0.547±0.002).Compared with the the model group(0.780±0.004),the difference was obvious(p<0.01),with the normal group and positive group showed no significant difference.High dose acteoside the number of rat prostate gland(21±2)was closed to the normal group,significantly less than the model group and positive drug group(p<0.01),showed that treatmented high dose acteoside the rats prostate gland returned to normal.Under the microscope,the normal rats prostate gland are evenly distributed (18±2),epithelial cells were single neatly arranged,gland cavity size was uniform,there were some secretions in gland cavity.In model group,the number of glands(25±1)were increased and intensive,the epithelium papillary uplift,cavity was extended, the high cylindrical gland cells showed complex layers,interstitial was increased. The number of positive group rats prostate gland(21±2)significantly more than the normal group,the prostate gland cavity was obvious expansion. The number of high dose acteoside group rats prostate gland(21±2)significantly less than the model group and positive drug group(p<0.01), epithelial cells with a single layer,neatly arranged,mesenchymal uniform, glandular secretions cavity with visible red, close to the normal group prostate cell structure.The high dose acteoside improved rat BPH,and the improvement effect was superior to positive drug.②AR positive expression that stainned brown particles were located on the epithelial cells. In normal rats prostate AR expression was weakened.AR expression markedly enhanced in model group(5.702±0.187)% ,compared with the normal group(3.149±0.117)% there was significant difference(p<0.01).For the middle and high dose acteoside weakened the AR expression on prostate epithelial cells (4.481±0.31)%,(3.498±0.524)%,compared with the model group there were significant differences(p<0.01),with the normal group showed no significant difference.In the high dose acteoside group(3.498±0.524)% AR expression was weaker than the positive group(4.661±0.455)%,there was significant difference(p<0.01).Rats prostate TGFβR1 positive staining was reddish brown in the epithelium and Mesenchymal. In normal rats prostate TGFβR1 expression was weakened(9.91±0.496)% , the model group TGFβR1 expression was significantly increased(14.75±1.126)%,compared with the normal group(9.91±0.496)% there was significant difference(p<0.01). The middle and high dose acteoside weakened the TGFβR1 expression(10.98±0.478)%,(10.046±0.297)%,compared with the model group there were significant differences(p<0.01),with the normal group showed no significant difference;the high dose acteoside group(10.05±0.297)% TGFβR1 expression was weaker than the positive group(11.30±0.531)%,there was significant difference(p<0.01). Comprehensived above all that high dose acteoside weakened AR and TGFβR1 expression,which was stronger than positive drug.③FCM found that the normal rats prostate apoptosis rate was very low,may be related the young rats.Treatmented high middle and low dose acteoside respectively,the rats prostate cells apoptosis rate(24.70±1.85)%,(6.1±1.04)%,(14.5±0.68)% were significantly higher than the normal group(1.10±0.06)% model group(9.50±0.5)% and positive group(9.83±0.76)%,there were significant differences(p<0.01).It showed that acteoside could induce prostate cells apoptosis,and stronger than positive group .④Transmission electron microscopy showed normal cell integrity and nuclear chromatin evenly distributed.RER was rich and secretions could been seen in cavity.There were deformat nucleus of prostate cells,rough endoplasmic with cystic high degree of expansion and marked vacuoles in model group prostate.High dose acteoside prostate nuclear chromatin density increased,the endoplasmic reticulum to reduce cavitation,could found apoptotic bodies.Conclusion Acteoside could adjust endocrine function of the prostate,inhibit rat begnin prostatic hyperplasia.Acteoside inhibited the rat experimental BPH model maybe by downing AR and TGF-βR1 expression,inhibiting prostate epithelial cell proliferation and promoting prostate cells apoptosis.
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