The Expression Of Skp2 And P27 In Oral Verrucous Carcinoma

Objective:To study the expression of Skp2(S-Phase kinase associated Protein 2)and cyclin-dependent kinase inhibitor p27 in the oral verrucous carcinoma(OVC)and their relationship with clinicoPathologic characters,so as to provide a new way for early diagnosis,prognosis assessment and treatment of OVC.Besides,the results were compared with those of oral squamous cell carcinoma (OSCC)to discuss their difference and relationship.Methods:The immunohistochemistry technic was used to detect the expression of Skp2 and p27 in 15 OVC tissues,30 oral squamous carcinoma(OSCC)tissues and 15 normal oral mucosa tissues.Statistical analysis was performed by SPSS 12.0 software.Results:1.The expression of Skp2 The expression rates of Skp2 in the OVCs and OSCCs were significantly higher than those in normal mucosa (P＜0.05),but there was no difference of Skp2 expression among the OVCs and OSCCs(P＞0.05).In the OVCs,There was no difference of Skp2 expression in sex,age of the patients,as well as the tumour site or stage(P＞0.05).For different phenotypes of OVC,the positive expression rate samples were different(P＜0.05),while in the OSCCs,Skp2 expression was significantly associated with stages,differentiated grades and lymph node metastasis.The samples with higher stage,poorer differentiated grade or more probability lymph node metastasis had a significantly higher expression of Skp2.2.The expression of p27 The expression rates of p27 in the OVCs(43.5%)and OSCCs(53.3%)were significantly higher than those in normal mucosa(93.3%)(P＜0.05).But there was no difference of p27 expression among the OVCs and OSCCs(P＞0.05).In the OVCs,there was no difference of p27 expression in sex,age of the patients,tumour site,or stage(P＞0.05).The positive expression rate samples of different types of OVC were different(P＜0.05),while in the OSCCs,p27 expression was significantly associated with differentiated grades and lymph node metastasis,p27 staining in poorly differentiated carcinoma or more probability lymph node metastasis is remarkably reduced.3.Spearson anlysis showed that Skp2 was inversely associated with expression of p27 in OVC.(r₁=-0.935,P<0.01),and Skp2 was inversely associated with expression of p27 in OSCC.(r₂=-0.639,P＜0.01)Conclusions:1.The expression of Skp2 and p27 may contribute to the carcinogenesis of OVC and OSCC,and has close relationship with the malignant potential of tumors.2.Skp2 and p27 may contribute to the progression and metastasis of OVC and OSCC.3.Skp2 plays an important role in the down-regulation of p27 during oral cancer development.