| ObjectiveTo study the antiemetic efficiency of extractions from capsicum and gingers and investigate potential antiemetic mechanism of synergistic actions, in order to develop traditional sapid substances into natural drugs which are hypotoxic and effective.Method1. To purchase high concentration and fine quality extractions from gingers and capsicum, main contents separately are gingerols and capsaicin2. After the emetic models of rats are successfully established, rats were randomly divided into various groups (six animals per group) , and administrated with different dosages of cisplatin, apomorphine, rotated(100 r·min-1, 1h), then the consumption of kaolin was measured, antiemetic effects of mixture were evaluated.3. On the basis that emetic models of minks had already been successfully established, minks were randomly divided into various groups (six animals per group), and given different dosage of admixture of extractions and positive control drug, then the antiemetic effects of admixture were observed.4. By hematoxylin-eosin staining method, pathological sections were made from rats' gastric wall in control and antiemetic reagent groups, for observing hemorrhage condition in muscular layer of gastric wall.5. By immunohistochemistry the contents of Substance P, which was in the tissue of sinus ventriculi of rats from the control groups and other groups, were measured. According to the expression of Substance P, the relationship between SP and this experiment was evaluated, meanwhile, the synergistic actions of gingerols and capsaicin present or not can be initially observed.Results1.In cisplatin (3.0mg·kg-1 ),apomorphine (3.2mg·kg-1 )and rotation situation(100r·min-1, 1h) groups, the rats expressed eating non—nutrition substances kaolin ,called pica ,compared with the control group. P<0.01 2. Admixiture can oppress the pica behavior in rats induced by nauseants such as cisplatin , apomorphine and rotation .3. The extractions from ginger and capsicum (gingerols and capsacin) can inhibit emesis induced by nauseants such as cisplatin, copper, sulfate, apomorphine and motion sickness in minks .Emetic latency was increased while the frequency of retch and emetic episodes were decreased compared with the control group (P<0.05)4. Pathological sections of rats' gastric wall demonstrated, compared with the control group,the quantity of hemorrhage in antiemetic reagent groups is small,especially in large-dosage groups hemorrhage significantly decreased. This certified the mixture to some extent had the fuction of protecting the gastric mucosa.5. The result of immunohistochemistry test revealed that, compared with the positive drug group, the reactions of postive neurofibra in the muscular layer of sinus ventriculi from antiemetic reagent groups were significantly diminished and the tinction was light.Conclusion1. The rats' pica models are successfully established and pica behavior can be inhibited by the extractions from ginger and capsicum (gingerols and capsacin), to some extent, mixture can protect the gastric mucosa.2. The mixture (gingerols and capsacin)can significantly inhibit the emesis in minks.3. The potential mechanism of antiemetic synergistic actions may correlate with SP and 5-HT. |
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