Study On Serum Protein Fingerprint Related To Anxiety By SELDI In Cancer Patients

Object: Using SELDI-TOF-MS technology to screen the serum protein fingerprint related to anxiety in a group of cancer patients and develop diagnostic patterns.Compared with the results of Hamilton Anxiety Scale in cancer patients.then evaluate its clinical significance.Methods: 125 cases cancer patients were collected in this experiment.They were investigated by the Hamilton Anxiety Scale first,all the patients were divided into two groups(anxiety group and non-anxiety group) by the standard of≥14 scores, then the SELDI-TOF-MS technology and the CM10 protein chip were used to screen the serum protein fingerprint related to anxiety.Using the 5% protein fingerprint peak as the diagnosis model and HAMA as the gold standard to evaluate the correct ratio,sensitivity, specificity, positive predict value and negative predict value of this method.Results:①125 cases cancer patients were investigated by the Hamilton Anxiety Scale,49 cases of the cancer patients were judged as anxious patients and the other 76 cases were the non- anxious patients by the standard of≥14 scores,the occurrence rate is 39.20%;②In patient's serum protein examination, at the M/Z values range from 15000Da to16800Da,there were obviously different protein fingerprint between the serum of anxious patients and the serum of non-anxious patient.There was a positive correlation between the results of the serum protein fingerprint and the Hamilton anxiety scale(P=0.001,r=0.809). This serum protein fingerprint can be regarded as a specific serum biomarker to anxious cancer patients;③The diagnosis model of 5% protein fingerprint peak could be utilized to analyze protein profiling in screening anxious-related cancer patient.The correct ratio(total coincidence rate) is 93.27%,(111/119),the sensitivity and the specificity respectively are 93.61%(44/47), 93.05% (67/72).The positive predict value is 89.79%﹝44/49﹞,the negative predict value is 95.71%﹝67/70﹞.Conclusion: SELDI-TOF-MS technology can be used quickly and exactly in the detection of the serum protein fingerprint related to anxiety in cancer patiets. At the M/Z values range from 15000Da to16800Da,5% protein fingerprint peak can be regard as Anxiety Protein Fingerprint. with high sensitivity and specificityTthis method will be widely used in clinical application.