Effect Of United Micronutrients On Antioxidative Status And The Expression Of Cytokines In Diabetic Mice

BackgroundType 2 diabetes is a common disease of endocrine and metabolism. Along with the progress of the social and people's living standard, the incidence rate rise increasingly. Diabetes is a incurable disease, and the main hazards is its complications, so the quality of diabetic patients'life is reduced largely. Currently, the treatments of DM program are mainly in controlling high glucose. Including oral hypoglycemic drugs, insulin and ananogues, Wide varieties of DM drugs are developed at home and abroad. Including the agents of promoting secretion of insulin, the agents of regulating insulin secretion pattern, the insulin sensitizing agents, inhibiting the production of glycogen and releasion of the glucose, and inhibiting the formation and absorption of glucose in intestinal, even combined using drugs which have multidimensional and synergistic effect. These classic drugs can effectively control high blood sugar, but there are obviously negative effect if long-term use. Therefore, explore new therapeutic target which combined with the pathogenesis of DM is very necessary.ObjectivesTo investigate the effect of Yeast-Zinc, chromium, selenium united antioxidant micronutrients (YAM) on antioxidative status, immune function, on the expression of cytokines genes and on the pathology structure of pancreas and liver in experimental diabetic mice, and to provide a scientific basis for the use of it in preventing diabetes and its complications.MethodsTwenty mice were randomly selected from 100 male Balb/c mice and were divided into normal control group and negative control group (normal mice + high dose YAM), each group have 10 mice, and the remaining 80 mice were given intraperitoneal injection of one-time high-dose STZ (150 mg/kg STZ) to establish diabetic mice model. After modling success, the diabetic mice were randomly divided into four groups, named as diabetes model group, YAM low (L), medium (M), high (H) dose group. Each dose group was treated by gavage for eight weeks, then get the blood in picking eyeball and then cervical dislocation, separating serum, measuring blood glucose, blood lipids and superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione (GSH), the cells of malondialdehyde (MDA), alanine aminotransferase (ALT) in serum. Get the liver, kidney and brain of mice, measuring SOD, GPX, GSH, and MDA in them. Get the liver and pancreas of mice, observing changes in pathology. Gets the spleen of mice, homogenating, measuring the expression of lymphocyte IL-2, IL-4 in the spleen by flow cytometry.Results1. The YAM could significantly delay the upward trend of diabetic mice's glucose, and the middle and high group have significant effect (P<0.05).2. The YAM could reduce TC, TG,LDL level of diabetic mice ,and raising HDL level (P<0.05),which dedicated the recovery of the lipids in diabetic mice.3. The YAM could reduce MDA, and raise SOD, GPX, GSH level of diabetic mice (P<0.05) in serum, which dedicated the improvement of oxidative stress in diabetic mice.4. The YAM could reduce MDA, and raise SOD, GPX, GSH level of diabetic mice (P<0.05) in liver, kidney, and brain, which dedicated the improvement of oxidative stress in important organs in diabetic mice.5. The YAM could ease the injury of pathological change in the liver and pancreas islet, which dedicated it can protectβ-cells in diabetic mice.6. The high dose YAM have no significant effect on ALT in normal mice, which showed the subject have no harmful effect on mice's liver cells. Combining with the HE of liver, which dedicated it have protective effect on liver cells.7. The YAM could increase the level of thymus coefficient, spleen coefficient (P<0.05), and increase the expression of IL-2, IL-4 in spleen lymphocytes in diabetic mice (P<0.05), which show it can reduce the inflammatory response in mice in some extent, and strengthen the diabetic mice's immunity function.ConclusionsYAM can delay the upward trend of diabetic mice's glucose, antagonize the oxidative damage significantly, ease inflammatory response, and increase the body resistance. At the same time, the high dose YAM have not any injury in normal mice, which may predicted the important effect of YAM on easing the symptoms and complications of diabetes, and improving body resistance.