The Expression Of Programmed Cell Death 4 And Ki67 Protein In Mycosis Fungoides Of Different

ObjectiveTo explore the expression of the Programmed Cell Death 4 and Ki67 antigen in the skin infiltrates of normal skin,skin lesions with mycosis fungoide (MF) in different stages and lichen planus(LP);And to explore the significance of the human programmed cell death 4 and Ki67 in MF.Methods1.27 cases diagnosed by clinic and pathology as granuloma fungoides in the last ten years in the department of Dermatology of QiLu Hospital were collected. As normal control,5 normal skin specimens obtained from clinic Operating Room and Plastic Surgery,were fixed by formaldehyde and embedded by paraffin.As inflammation control,5 lichen planus were obtained from Pathology Room of Dermatology.2.The expression of PDCD4 and Ki67 in epidermis and dermis was detected by immunohistochemistry technique.3.By using JMTJFX concise statistical analysis software 10.34,the expression of protein PDCD4 and Ki67 in epidermis and dermis was tested by rank-sum test of multisample by unitizing engineered or analysis of variance;The relevance of the two protein was analysed by Spearman'S rank correlation,the statistically significant difference is P＜0.05. Results1.Location and intensity of PDCD4 in immunohistochemical stainNormal control specimens were all strong positive,mostly with brownish palm color,and positive expression was located mainly in the nuclear.In LP,the keratinocyte and lymphocyte in dermis were widespreadly positive with buffy almost in nuclear,intense distribution.In erythema/plaque stage MF,slightly decreased positive cells with buffy or pallide-flavens in epidermis were observed and the positive expression was located in both nuclear and kytoplasm;obviously decreased positive cells in dermis with buffy or pallide-flavens were observed in both nuclear and kytoplasm.In tumor stage MF,significantly decreased positive cells in epidermis and dermis stained mostly in kytoplasm with diffused distribution,partly absent in dermis.2.Comparison of positive expression intensity among all groups(PDCD4).In epidermis,from normal control,erythema/plaque stage MF to tumor stage MF,or From LP,erythema/plaque stage MF to tumor stage MF,the expression of PDCD4 weakened gradiently.There was no significant difference in the number of PDCD4~+ cells between patch/plaque stage MF and normal control or LP(P＞0.05), and there was significant difference among the rest groups(P＜0.05).In dermis,from normal control,erythema/plaque stage MF to tumor stage MF,or From LP,erythema/plaque stage MF to tumor stage MF,the expression of PDCD4 weakened gradiently.There was no significant difference in the number of PDCD4~+ cells between patch/plaque stage MF and LP(P＞0.05),and there was significant difference in the rest groups comparison(P＜0.05).3.Location and intensity of Ki67:all in nuclearPositive basal cell with buffy in normal control was partially observed;and negative in dermis.In LP,the positive basal cells distributed discontinuously,the expression decreased where there were liquefaction of basal cells and increased in other place. Positive cells with buffy in dermis were more and intense. In erythema/plaque stage MF,positive cells with huffy decreased in basal layer and slightly increased in epidermis,while scattered in dermis.In tumor stage MF,positive cells with brown in epidermis scarcely increased while were intense in dermis,and cells obformed with karyomegaly anachromasis.4.Comparison of positive expression intensity among all groups(Ki67)In epidermis,there were no significant differences among all groups.In dermis,from normal control,erythema/plaque stage MF to tumor stage MF, the expression of Ki67 gradiently increased and there were significant difference among group comparison(P＜0.05).There were no significant difference between LP and erythema/plaque stage MF or LP and tumor stage MF(P＞0.05),while there was in latter two group comparison(P＜0.05).5.correlation analysis between PDCD4 and Ki67:There was on correlation of PDCD4 and Ki67 either in erythema/plaque stage MF or in tumor stage MF(P＞0.05).Conclusion1.The expression of PDCD4 in epidermis keratinocyte,with the progression of MF,obviously down regulated even absent,indicates that keratinocyte may play an important role in the pathopoiesis and progression of MF.But the detail role needs further investigation.2.The expression of PDCD4 in infiltrating lymphocyte of dermis,with the progression of MF,obviously down regulated even absent,indicates that PDCD4 intimately correlates with the pathopoiesis and progression of MF.It may contribute to inhibit excessly proliferation of lymphocyte by up-regulating the expression of PDCD4,and PDCD4 is expected to be a new target for treating CTCL.3.The expression of Ki67 in normal control,erythema/plaque stage MF and tumor stage MF rises up gradually,which suggests that Ki67 antigen relates to the proliferation of lymphocyte in dermis,but there is no significant difference with LP.So we consider that Ki67 alone can't be the criteria to judge if cells proliferate malignantly. 4.No correlationship was observed between the expression of PDCD4 and Ki67, which suggests that apoptosis disturbance led by PDCD4 does not directly affect cells proliferation associated with Ki67.