Influence Of Jiaweibugan Decoction On JNK And C-jun Expression Of Sciatic Nerve In Experimental Diabetic Rats

Objective:to observe the effect of jiaweibugan decoction on the expression of p-JNK mitogen-activated protein kinase and c-jun in sciatic nerve of experimental diabetic rats and explore the preventive and therapeutic effects of Jiaweibugan Decoction on peripheral neuropathy on experimental diabetic rats.Methods:1.Randomly selected 20 rats from 65 clean grade male Wistar rats as normal control group,and remnant 45 rats were established by streptozotocin(STZ) as diabetic model.The 40 rats which were successful induction were randomly divided into two groups including model-control group and JWBG decoction-treated group.2.Nonfasting plasma glucose and weight were measured on 4^th,8^th week and before treatment respectively.3.The rats were killed on 4^th or 8^th week from the beginning of treatment respectively:the blood serum and sciatic nerve were taken out, and then various parameters were measured as follows:serum SOD was determined by xanthine oxidasemethod;p-JNK expression of protein in sciatic nerve by immunohistochemistry;c-jun mRNA expression by reverse-transcriptase polymerase chain reaction(RT-PCR),and detecting the speed of conduction in sciatic nerve before being killed respectively. Results:(1)The effects of JWBG decoction on blood glucose and weight: compared with the model group,JWBG decoction has no significantly ameliorating to weight and blood glucose of the rats with diabetes induced by streptozotocin(P＞0.05).(2)The effects of JWBG decoction on the sciatic nerve conduction velocity:compared with the normal group,the sciatic nerve conduction velocity of the model group is significantly slow on the 4^th and 8^th week after successful induction by STZ(P＜0.01).Intervention with JWBG decoction-treated group could slower the decreasing of nerve conduction velocity,and at the same time there also are a significant difference between the JWBG decoction-treated group and model group(p＜0.05).(3) The effects of JWBG decoction on serum SOD activity:The results showed that SOD in the model group on 4^th or 8^th week was lower than that in the normal control group(P＜0.05),and it became much lower with diabetic course prolonging gradually,while which in jiaweibugan decoction intervened group was markedly higher than that in model rats(P＜0.05).(4) The effects of JWBG decoction on the expression of p-JNK mitogen-activated protein kinase and c-jun in sciatic nerve:The results showed that the expression of p-JNK and c-jun in the model group on 4^th or 8^th week was higher than that in the normal control group (P＜0.05),while which in jiaweibugan decoction group was markedly lower than that in model rats(P＜0.05).Conclusion:1.Jiaweibugan decoction could up-regulate the activity of SOD,and have the effect of anti-oxidative stress.2.JWBG decoction down-regulate the expression of p-JNK, inhibiting transcription of c-junmRNA and decreasing apoptosis may be through adjusting the oxidative stress state of organism,thus it can protect nerve cell from oxidative injury.This may be one of the function mechanisms of protective effects of Jiaweibugan Decoction on diabetic peripheral neuropathy.