| ObjectivePatho-based study of serum procalcitonin and Candida albicans in rats the relationship between the degree of bronchial lung infection.MethodsFirst,the establishment of animal models of Candida albicans pneumonia, Select male SD rats weighing close to18,Random into immune suppression infection group(immune suppression+White beads bacterial infection),Immunization normal infection group(immunization normal+White beads bacterial infections), Immunosuppressive non-infected group(immune suppression+physiological saline),6 in each group,Each group were randomly sorted.immune suppression infection group and Immunosuppressive non-infected group subcutaneous injection of hydrocortisone the establishment of animal models of immune suppression.immune suppression infection group and Immunization normal infection group intratracheal injection of immune 0.2ml containing 1-5×106cfv/ml the standard strains of Candida albicans suspension,Made of rat animal model of Candida albicans pneumonia. Immune suppression in the same way the control group normal saline was inoculated.The three rats 24 hours after inoculation, respectively, the first 1,3,5,7 daily tails Extraction of blood serum taken,Separation of serum,-30℃to save.The 7th uniform death rat,Separation of rat right lung lower lobe,Above 4% paraformaldehyde fixed 48 hours,Pathological sliced HE dyeing and observer organizations lung pathological changes.Serum treatment:The law ELISA testing serum PCT content,Observation,analysis of these three groups of animals,and changes in serum levels of PCT,Compared with the pathological changes.Research white rats beads bacteria bronchial lung infections and blood levels of calcium in the quality of the relations between. Results1.Three groups of pathological changes in rat lung tissue:Immunosuppressive non-infected group:The naked eye pink, Smooth surface, Sharp edge of thin, Touch of the homogeneous, No induration. Mirror the air sacs clear structure, Alveolar clear, and no congestion, congestion and cellular infiltration; Immunization normal infection group:Visual observation of lung tissue visible on the surface of white lesions of different sizes, Surrounded by hyperemia of the flush, the soft touch no induration. Mirror bronchial around the lungs of the Organization first necrosis small stoves, some air sac off the skin, lung organizations around inflammatory response clearly; immune suppression infection group:The naked eye can see uneven surface of lung tissue, a number of white lesions of different sizes, quality and tough, the surrounding tissue hyperemia. Endoscopic lung tissue various focal necrosis within the area, boundaries not clear enough, there is convergence between the lesions and alveolar structure disappeared, the surrounding tissue consolidation and alveolar spaces and interstitial inflammatory cell infiltration can be seen.2.Rats in each group at different time and changes in serum PCT levels:immune suppression of serum PCT levels in infected group 274.97-339.69ng/L between the comparison by the first 22 days of serum PCT levels of 1,3,5There was no significant difference, (P> 0.05), The 7th day blood serum PCT level obviously is higher than 1,3, 5 day blood serum PCT level, (P<0.05);immune normal serum PCT levels in infected 263.12-279.96ng/L between the comparison by the first 22 days of serum PCT levels of 1,3,5,7 There was no significant difference, (P> 0.05); immune suppression uninfected serum PCT levels in 181.46-198.97ng/L between the comparison by the first 22 days of serum PCT levels of 1,3,5,7 There was no significant difference, (P> 0.05).3.Three different times of serum PCT levels of comparison:immune suppression and immune to normal infection group infected with Group 1,3,5,7 days of serum PCT was significantly higher than non-infected immunosuppressed group,(P<0.05); the first 7 days immunosuppressive serum PCT levels of infection were significantly higher than the normal immune infection group (P <0.05).Conclusion 1.Whether it is normal immune suppression is immune as long as the bacteria infection beads white rat 1,3,5,7 days PCT serum immunization levels are significantly higher infection control team did not.2.The 7th days immune suppression serum group PCT infection levels higher than normal infection immunization, coupled with lung organization pathological changes, the serum PCT levels and white beads bacteria level of bronchial lung infections...
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