Investigation Of Protectant Effect Of Glycyrrhiza Extract On Medium Wave Ultraviolet Ray(UVB) Radiated BALB/c Mice Skin

Objective:To investigate the protective effect of extractive of Glycyrrhiza, Rhodiola rosea L, AM in BALB/c mice skin irradiated by ultraviolet B (UVB). To study the influence of topical application of extractive of this tradition Chinese medicine on HSP27, HSP70 and IL-10 expression levels in the skin of BALB/c mice irradiated by ultraviolet B (UVB). To explore anti-UVB protective effects of extractive of this tradition Chinese medicine in BALB/c mice skin and the possible protective mechanism. To provide theoretical basis and experimental reference data for the natural sunscreen development and practical application.Methods:54 BALB/c mice were randomly divided into nine groups with back hair shaving, shaving area of the skin were topical used 5% or 10% water solution of Glycyrrhiza, Rhodiola rosea L,AM, respectively.500mJ/cm2 ultraviolet B (UVB) was irradiated after 20min. Manipulated in this way for consecutive 30d. The skin samples were obtained after one month. Visual observation the general change of back skin. Histology of skin samples was studied by HE staining. HSP27 and HSP70mRNA expression level was measured by RT-PCR. IL-10 level was measured by ELISA.Results:After UVB irradiated, erythema, pigmentation, thickened, scaling were seen on back skin of mice by visual observation. Thickening of the epidermis and inflammatory cell infiltration in the dermis were observed by HE staining. Different doses is only a slight thickening of the skin after treatment by Glycyrrhiza,no scaling,pigmentation, morphology closer to normal tissue; different doses of slight thickening of the skin after treatment, there scaling, pigmentation, morphology some improvement by Rhodiola rosea L and AM; HSP27 and HSP70mRNA expression levels in the skin of irradiated BALB/c mice was statistically increased compared with the control group(P< 0.05), The pretreatment of Glycyrrhiza,Rhodiola rosea L,AM could raise regulate HSP27 and HSP70mRNA expression levels induced by UVB irradiation but there was no definite dose-dependentce compared with the control group(P> 0.05), IL-10expression in the skin of irradiated BALB/c mice was statistically increased(P<0.05), The pretreatment of Glycyrrhiza,Rhodiola rosea L,AM could raise regulate IL-10expression levels induced by UVB irradiation but there was no definite dose-dependence(P> 0.05).Conclusion:Skin photoaging phenomenon was effectively improved by topical application of Glycyrrhiza,Rhodiola rosea L,AM extract solution, when the effect of topical application of aqueous Glycyrrhiza extract was more obvious. It may be related to induced HSP27, HSP70 and IL-10 expression of the three kinds of traditional Chinese medicine.