Study On The Relationship Between Vascular Endothelial Growth Factor-C, Vascular Endothelial Growth Factor-D And Metastasis Of Lymph Node In Gastric Carcinoma

Background:gastric carcinoma is one of the most common cancer of mankind。its invasion is a Pathobiology process of multi-factor, multi-gene and multi-steps. Globally, gastric carcinoma ranks first in Cancer while its mortality is in the first place. it is an important way for metastasis of gastric carcinoma by lymphatic spread. The metastasis by lymphatic spread happened earlier than that did by blood vessels. It is found that metastasis already had happened in about 50% patients at diagnosis. Lesion invasion and metastasis of lymph node have an important impact on the prognosis and they are the leading causes of death in patients. Therefore, the research on the pathogenesis of infiltration and metastasis and their basis of molecular biology is significant to clinic for controlling metastasis of gastric carcinoma and improving survival in patients. In recent years, it is discovered that vascular endothelial growth factor(VEGF) plays a very important role in tumor growth and metastasis. Now, VEGF has become one of the important foundations of the research on molecular mechanism of tumor metastasis. Especially, the role of VEGF in metastasis of lymph node has become one current research focus. It is found that VEGF-C and VEGF-D, both of subtypes of VEGF and highly homologous, are closely related with clinicopathological features and prognosis of various tumors. They are major regulatory factor in generation of lymph vessel and are important in metastasis by metastasis of lymph node. However, in present, there are less reports on the impact of VEGF-C and VEGF-D on metastasis of lymph node of gastric carcinoma. And Such kind of impact is not agreed unanimously because certain difference are showed among results of study on the roles of VEGF-C and VEGF-D in lymph node metastasis of gastric carcinoma. Objective:This subject is for further study on the expression of VEGF-C and VEGF-D in tissues of gastric carcinoma and on the role of VEGF-C and VEGF-D in metastasis of lymph node.Methods:We classified 94 cases which were treated by gastrica denocarcinoma tomy into two groups (negative and positive for lymph node metastasis), and 41 cases of normal gastric tissues were used as control group. The expression of VEGF-C and D protein in gastric carcinoma tissues obtained from 94 patients who under went radical gastric tomy and in normal gastric tissue of 41 cases were detected by immunohisto-chemistry.Results:VEGF-C can be stained in the cytoplasm of the gastric carcinoma tissues and nomal gastric tissues by immunohistochemistry. The positive rates of VEGF-C in gastric carcinoma were much higher than that of control group, There was a correlation between the expression of VEGF-C (P<0.05) The positive rates of VEGF-D in gastric carcinoma were much higher than that of control group (P<0.05).There was a significant correlation between the expression of VEGF-C and lymph node metastasis (P<0.05). There was a significant correlation between the expression of VEGF-D and lymph node metastasis. but There was not a correlation between the expres-sion of VEGF-C (P>0.05). Logistic regression analysis also showed the correlation of the VEGF-C expression in gastric carcinoma with lymph node metastasis (OR=1.944, Standard Estimate=-0.665, P<0.05).Conclusions:The positive rates of VEGF-C is much higher than that of control group and the expression of VEGF-C is closely related to lymph node metastasis in gastric carcinoma. The positive rates of VEGF-D is much higher than that of control group, it might be involved in tumor progression and can not indicate the metastasis by lymph node.