Study Of Relationship In PCNA, DNA And AgNORs Of Colorectal Cancer

Objective: To investigate the expression of Proliferating Cell Nuclear Antigen(PCNA) and its Relationship with DNA Content and AgNOR Counts in Colorectal cancer .Methods: Using 42 Specimens of colorectal cancer which has been diagnosed by the pathologists of Guangxi medical university during 2007 and 2008.. Four cases of them are well-differentiated cancers , 24 cases moderate differentiated carcinoma, 14 cases are low differentiated carcinoma.And there are 20 cases of adenoma specimens, 10 cases are normal colon tissues. All the specimens are fixed by formalin , conventional HE dyeing , using immunohistochemical technique to examine the expression of PCNA and image analysis technique to study the DNA content and AgNOR counts, using software SPSS 12.0 t test to statistics.Results: The results showed that PCNA proliferating activity was confined to the nucleus of cancer cells.The labelling index ranged from 3.9-89.6%(median:33.68%).PCNA LI can devide into three grade:gradeâ… (0-25%)14cases,gradeâ…¡(26-75%)25cases,gradeâ…¢(76-100%)3cases.PCNA LI in lower differentiation , middle differentiation and higher differentiation is 61.6+/- 16,38,36.5+/-7.87,10.7+/- 7.12. The PCNA LI showed a correlation with size,differentiation of tumor and prognosis of patients and no relationship with age,gender.There are also relationship in DNA content and AgNOR counts with differentiation of tumor and prognosis of patients.Furthermore,the expression of PCNA significently correlated with DNA content and AgNOR counts in colorectal carcinoma.Conclusions: The results suggest that a combining study of these three indexes may be more accurate in detecting the proliferating stages of cancer cells and there will be more value in assessing biological behavior and the prognosis of colorectal carcinoma...