Study On The Early Liver Metastasis Forecast Of Colorectal Tumor By Associate Detection Of CK20mRNA, CD44v6 And PCNA

[Object] To obtain some effective objective markers to predict the early hepatic metastasis ,we explore the relationship between CK20mRNA, CD44v6, PCNA and hepatic metastasis from colorectal cancer. [method] The expression of CK20mRNA in portal vein blood from 50 colorectal cancer patients was detected by fluorescent quarto RT-PCR, and CD44v6, PCNA in colorectal cancer tissue were determined by SP method of immunohistochemistry. Its relationship with hepatic metastasis were analyzed, and then compared with control groups through statistics analysis. [Result] The rate of positive expression of CK20mRNA in colorectal cancer patients' portal vein blood was 62%, and obviously higher than control groups (p<0.05). The rates of positive expression of PCNA and CD44v6 in colorectal tumor tissue were respectively 62%,64%, and distinctly superior to the level control groups (p<0.05) . These three markers were related with Dukes (P<0.05) ,and the positive expression rate of C+D period is clearly higher than that of A+B period (p<0.05) , there was no correlation between them and the differentiation degree of cancer tissue (p>0.05). The rates of positive expression of CK20mRNA,CD44v6 and co-expression of the three markers in hepatic metastasis patients were evidently higher than those of no hepatic metastasis (P<0.05) . The rate of positive expression of CK20mRNA in portal vein blood was evidently correlated to the expression of CD44v6 and PCNA in tumor tissue (P<0.05) . The sensitivity and specificity forecasting hepatic metastasis from colorectal cancer were 80.6%~92.3% and 45.9%~48.6% by analyzing the single expression;however, analyzed the co-expression of the three markers, the sensitivity and specificity were respectively 53.8% and 78.3%. [Conclusion] CK20mRNA, CD44v6 and PCNA are correlated with the risk of developing hepatic metastasis from colorectal cancer. The co-expression of these markers has very high sensitivity and specificity, and they are important clinical significance in predicting hepatic metastasis from colorectal cancer.