The Changes Of ICAM-1 Expression Of Obstructive Jaundice Post-released In Animal Experiment

Objective:To investigate the changes of the expression of intercellular adhesion molecule 1 (ICAM—1) in WBC, liver, distal ileum of obstructive jaundice post-released in SD rats. And to assess relationship between the increased ICAM-1 expression and obstructive jaundice.Methods:One hundred and twenty adult Sprague-Dawley rats were randomly assigned to three groups:sham operation(SH, n=40),obstructive jaundice(OJ, n=40) internal biliary drainage (ID, n=40). SH group, only the first separation surgery of common bile duct, OJ group, ID group underwent bile duct ligation. After seven days, A second surgery, SH groups to deal with the former, OJ group only isolated Common bile duct, ID group underwent biliary enteric drainage. On the 7th after the first operation and 1st,3 th,7th days after the second operation, the three groups were succumbed and the specimens of blood and liver, distal ileum were collected. The level of total bilirubin(TB), albumin (ALB), alanine transminase (ALT), aspartate aminotransferase (AST) in serum and liver, distal ileum tissue to value the result of the operation.The expression of ICAM-1 on WBC, liver, distal ileum tissue at different time points was detected by flow cytometry(FCM). HE conventional pathological section with the liver,intestine and other histopathological and immunohistochemical observation of intracellular adhesion molecule 1 (ICAM-1) in the liver, intestinal tissue, on the data for statistical analysis.Results:1. The level of TB, ALT, AST,were rising and reaching the peak on the 7th day after the bile duct ligation. Ligation group and the SH group:P<0.05, were statistically significant, suggesting that the biochemical indicators increased significantly on the 7 days after biliary obstruction. The biochemical indices of ID group underwent biliary enteric drainage decreased significantly, and returned to normal on the 7 day after surgery. The biochemical indices of OJ group did not remove the obstruction, continuing high levels.The two groups at different times after the second operation compared:P<0.05 were statistically significant, and indicated that the biochemical decreased significantly after biliary enteric drainage.2. The tissue cells of normal rats showed a low expression of ICAM-1 levels.The cell ICAM-1 levels were significantly higher than the SH group on the 7 days after the bile duct ligation. The ICAM-1of ID group underwent biliary enteric drainage decreased significantly. The ICAM-1of OJ group did not remove the obstruction, continuing high levels. The two groups at different times after the second operation compared:P <0.05 were statistically significant and indicated that the ICAM-1 decreased significantly after biliary enteric drainage.With the bile duct ligation prolonged the statistics is more significant; Prompted the ICAM-1 and liver, intestinal tissue injury was positively correlated3.The pathological changes of liver,intestinal:SH group showed normal lobular architecture of liver pathology. On 7th day after bile duct ligation, new small biliary epithelia cells appeared in the portal areas and around the liver lobulars with some inflammatory cells infiltrated; As epithelial cells of intra-hepatic bile duct exfoliated some necrosis focuses of hepatic cells were being seen. Pathological changes of ID group gradually returned to normal after biliary enteric drainage. On 7th day after the second operation, in OJ group, the hyper-plastic areas of intra-hepatic bile duct epithelial cells enlarged, the cells were flat and the lumens of the bile ducts were wide, the basal membrane and collagen fibers layers thickened. Occasional ductular epithelial cells can be divided into small bile ducts, proliferation of small bile ducts and fibroblasts, collagen fibers interval, split, converted the original lobule, the remaining liver cells to form large or small island structure. The rats of SH group, intestinal mucosa, gland morphology intact, Hair neat and no atrophic changes in the rules and no tissue necrosis of the performance. OJ group of intestinal mucosa can be found in the intestinal mucosa under light microscope observation of atrophy, villous edema, some epithelial cells shed, the average area of hair, height, the average thickness of the mucosa were reduced. With time mucosal obstruction thinner hair goes low, narrow, inflammatory cells increased, reduce the number of intestinal villi, shorter thicker, convergence, form a very irregular. ID group after the second surgery the pathological changes of intestinal tissue gradually returned to normal.Conclusion:By ligating the common bile duct causued the obstructive jaundice of SD rats.TB, ALT, AST, changes are different from humans. The level of TB, ALT, AST, were rising and reaching the peak on the 7th day after the bile duct ligation and then showed a slow upward trend. The biochemical indices of ID group underwent biliary enteric drainage decreased significantly, and returned to normal on the 7 day after surgery. The biochemical indices of OJ group did not remove the obstruction, continuing high levels.In obstructive jaundice, ICAM-1 cause the injury of liver,intestines and other tissue. The ICAM-1 of the liver,intestinal tissues and the levels of TB were positively correlated and suggested that the ICAM-1 have closely related with the time and level of obstructive jaundice; The leves of ICAM-1 and ALT, AST was positively correlated and indicated that with the ICAM-1 continued to rise, the aggravation of tissue injuryed. That all shows the increase of serum ICAM-1 is closely related with organ dysfunction, which can reflect the severity of tissue damage when the bile duct ligation. Detection of ICAM-1 can be used as an important indicator of obstructive jaundice (OJ) and suggested that early removed the Obstruction can reduced tissue damage.