Glucosamine Sulfate Regulates Differentiation And Matrix Gene Expression Of ATDC5 Cell

[Abstract] Objective:To investigate the effects of glucosamine (GlcN) on chondrocyte line ATDC5 proliferation, matrix production, and gene expression for providing insights into the biochemical basis of its reported beneficial effects in osteoarthritis (OA). Methods:Alkaline Phosphatase staining and Alcian Blue Staining were used to determine the effect of GlcN on cartilage matrix productioin. The impact of GlcN on gene expression was evaluated with real-time polymerase chain reaction (PCR). Results:Notably, GlcN enhanced cartilage specific matrix components, collagen typeâ…¡, inhabitant runx2 and Smad2. Conclusion:Glucosamine sulfate not only promote synthesis of cartilage matrix also affect the cartilage cells ATDC5 differentiation. [Abstract] Objective:Our objective is to evaluate whether the GDF5 core promoter polymorphism (+104T/C; rs143383) is associated with osteoporosis in the people of Chinese origin. Methods:The SNPs (+104T/C; rs143383) (in GDF5) was genotyped using a Taqman 5'allelic discrimination assay on an ABI 7500 real-time polymerase chain reaction (PCR) instrument in177 subjects who suffered from OP and 108control subjects, and analyzed their associations. Results:The genotype distribution and allele frequency were compared between OP and healthy control. Neither genotype distributions nor allelic frequencies of the assayed single nucleotide polymorphisms were found to be significantly different between patients and controls (P>0.05). Conclusion:Our results show that GDF5 does not seem to be a risk factor for osteoporosis etiology in Chinese Han population.