Advanced Development Of Carbon Nanotube-PVA-rh-EGF Combined Wound Dressing

ObjectiveDescribe a new wound dressing made of electrospun carbon nanotube and polyvinyl alcohol (PVA) containing epidermal growth factor (EGF). Observe its'general and microstructure characteristics. Investigate the release model of EGF from the wound dressing.Contents and methods1. Construction of the wound dressing:Acidify the carbon nanotube, mix the PVA and rh-EG with the acidified carbon nanotube and construct the non-woven wound dressing by using the electrospun technique.2. Observe the microstructure of the wound dressing through the use of a Scanning Electron Microscope (SEM).3. Invitro cytotoxicity test. Test the cytotoxicity of the wound dressing utilizing the MTT method on L929 cell. Groups were separated according to different density and time.4. Investigate the release model of rh-EGF from the wound dressing (invitro) by testing the density of rh-EGF at different times using the ELISA method.5. Evaluation of the biologic activity of the released rh-EGF from the wound dressing is done by using the MTT method. Groups were separated according to different times. Physiological saline solution is the control group and the OD-value of each test group was compared.Results1. SEM results show that the wound dressing has a stable microstructure.2. The OD-values of each test group were higher than the negative control group and that the cytotoxicity level for the wound dressing is 0. Thus indicating that the materials tested were safe.3. Rh-EGF has a sustained release rate from the wound dressing. The highest density of the rh-EGF was found 12 hours after the experiment began at 12.47% with a cumulative release rate of 9.4% after 24hours.4. The density of the rh-EGF sample is higher in the 12th and 24th hour's, therefore it has a stronger affect in accelerating the cell growth. The activity of the 12 hour experimental group and 24 hour group were 291.24% and 211.3%, which indicates the activity of the rh-EGF is stable after being released from the wound dressing.ConclusionThe carbon nanotube—PVA-rh-EGF wound dressing has a stable structure and has the ability to release the rh-EGF at a stable rate, with the activity of the rh-EGF release being acceptable.