Compression Bandage After Ice Process Simulation Experiment Research Of Athletic Soft Tissue Injury Effect

Rasearch Objective:Establish animal model of rabbit acute soft tissue injury to observe the acute soft tissue injury rabbit soft tissue changes; To compare different treatment methods for the treatment of acute soft tissue injury of rabbits, and explore the possible mechanism of action, can be engaged in sports and alleviate symptoms in patients with injury of soft tissue, accelerate the damaged tissue repair and functional recovery, and may not stay sequelae, avoid damaged tissue fibrosis, for the general public heal provides important reference basis for such diseases.Methods:Will be5months, weight2.02.5kg of40only male New Zealand rabbits, randomly divided into4groups, model group4, the normal group, control group, experimental group12in each group. Application of homemade heavy hammer blow device based soft tissue injury animal model, both legs on modeling. A is the normal group, not to blow mould, free food in the whole experiment, A normal life; B for the model group, blow mould, but not any damage processing; C in the control group, damage mode, and ice compression bandage after injury for processing; D group, damage to building and compression bandage ice damage after processing, free food in the whole experiment. All the rabbit conventional water and feed. After building, watch live at a different time change, measured with vernier calf hit point swelling degree, bruises and watch the symptoms, such as graded score. Model group damage after building timely injury muscle tissue, and blood from the heart, with a fully automatic blood rheology fast analyzer to detect all the indexes of hemorheology. The normal group, control group and experimental group3group of animals in building1d,3d,7d after three phase to be put to death animals, each group of each phase of four rabbit, take the blood from the heart5ml detection of all the indexes of hemorheology, and muscle group based in the area of the tag, drawn after application, eosin-wood grain (HE) staining observation changes of muscle fiber and inflammatory cells infiltration; Application of xanthine oxidase method for determining the content of superoxide dismutase (SOD), the application of glucosinolates barbituric acid (associates) method determination of malondialdehyde (MDA) of expression; Using immunohistochemical method, detection of interleukin10(IL-10) expression.Results:(1) Building the rabbit leg swelling rapidly after the phenomenon of bruises, skin and subcutaneous bleeding, injury within24h of injury is the most obvious. Id after injury, the control group and experimental group comparison (P>0.05), no statistical significance.7d after injury, the degree of swelling in the experimental group rabbits is lower than the control group (P<0.01), with very significant difference; Cutaneous petechial fading degree of the experimental group was higher than control group (P<0.01), with very significant difference.(2) The blood rheological index as a result, compared with normal group, model group after building in whole blood low cut, cut, cut high relative viscosity and plasma viscosity increased, erythrocyte pressure increased, showing a typical blood rheology characteristics of acute blood stasis syndrome.7d after injury, the experimental group whole blood viscosity low cut and high cut and red blood cell pressure were lower than the control group, with very significant difference (P<0.01), the experimental group in the whole blood viscosity and plasma viscosity lower than control group, with significant difference (P<0.05).(3) The histologic grading results, the normal group, control group, experimental group were lower than that of model group, has a very significant difference (P<0.01); In the same period in the control group compared with the experimental group, with significant difference (P<0.05).(4) Tissue homogenate method to detect superoxide dismutase SOD and malondialdehyde MDA expression, compared with normal group, model group, SOD activity decreased, MDA higher expression, with very significant difference (P<0.01); Over the same period compared to the control group and experimental group, SOD activity there was no significant difference (P>0.05), MDA expression had significant difference (P<0.05).(5) Immunohistochemical detection of the expression of IL-10, IL-10protein accumulation in muscle tissue absorbance values, the model group is lower than normal group, has a very significant difference (P<0.01); Control group, experimental group were higher than in model group, has a very significant difference (P<0.01); The experimental group and the control group, there was no significant difference (P>0.05).Conclusion:(1) In the rabbit model of acute soft tissue injuries, swelling and blood stasis, the respect such as color change mechanism may be related to aseptic inflammation, tissue repair and secondary fiber damage.(2) Ice compression bandage method and compression bandage after method can reduce the rabbit acute soft tissue injury of local muscle fibers swelling, degeneration necrosis, inflammatory cells infiltration, promote injury local muscle fibers regeneration, among them, the compression bandage after ice method is more effective.(3) After the compression bandage ice treatment can reduce the mechanism of rabbit acute soft tissue injury might increase and decrease MDA and SOD, increase the expression of IL-10, then reduce the aseptic inflammation, promote tissue fiber repair and reduce secondary injury.