Expression Of Glypican-3 Protein And C-myb Protein And Their Clinicopathological Significances In Primary Hepatic Carcinoma

Objective To investigate the expression of Glypican-3 (GPC3) protein and proto-oncogene C-myb protein in Primary Hepatic Carcinoma (PHC), and to explore the relationships of themselves and the correlation between them.Methods The specimens from 50 patients with Primary Hepatic Carcinoma (PHC) and 15 corresponding paraneoplastic hepatic tissues were fixed in 10% formalin and routinely embedded in paraffin. The specimens were continuously sliced into 3μm-thick sections. The expressions of Glypican-3 and C-myb were detected by immunohistochemistry SP method and scored under high-power microscopy.Results There are significantly statistical distinctions in the expressive positive rate and the expressive scores of Glypican-3 and C-myb protein between cancer tissues and paraneoplastic tissues (P<0.01, P<0.05)We haven't found the relatioships between Glypican-3 and main clinicopathological characteristics of PHC(P>0.05).There is no correlation between the expression of CAI protein and clinicopathological characteristics of PHC, except that the expressive positive rate and the expressive scores of PHC significantly higher with intrahepatic metatasis and tumor thrombosis than those which were without metastasis and tumor thrombosis.(P<0.05).There is no evident correlation between the expressive scores of Glypican-3 protein and that of C-myb protein in cancer tissues of PHC (r =0.23, P>0.05)Conclusion1. The high expression of Glypican-3 protein in PHC may play an impotant role in the diagnosis of PHC because of its sensitivity and especiallity.2. The expression of C-myb palys an important role in the invasive and metastatic part of PHC.It may be a harmful factor of prognosis.3. there is no correlation between the expressive scores of Glypican-3 protein and C-myb protein in cancer tissues of PHC...