Expression And Significance Of The WT1 Gene And Protiens In Pateints With Acute Myelocytic Leukemia

Objective:To explore the expression and significance of the WT1 gene and proteins in patients with acute myelocytic leukemia(AML).Methods:RT-PCR and immunohistochemistry were established to detect WT1 gene and proteins in mononuclear cells of bone marrow or peripheral blood in newly diagnosed and relapsing patients with AML, healthy comparisons and K562 cells. Then to observe the relationship of the expression of WT1 gene and proteins and therapeutic efficacy of AML.Results:The expression of WT1 gene and proteins in newly diagnosed patients with AML was respectively positive 38 in 45(86.7%) and 38 in 45(84.4%),in which 6 in 6 relepasing patients, and in control group was negative in all of the 16 persons. The complete remission rate in the patients whose WT1 gene or proteins expressed positive in newly diagnosed was 42.4% and 40.6%, which was 83.3% and 85.7% in the negative ones. The expression of WT1 gene and proteins in AML patients has a direct correlation relationship(r=0.277,P<0.05).The expression of WT1 gene and proteins have nothing to do with the patients'age, sex,onset peripheral blood cell counts and bone marrow blast cell count.There was statistically significant association between the relative level of WT1 gene expression and the achievement of complete remission. The relative level of WT1 gene≥1 indicates a poor prognosis in patients with newly diagnosed(/=4.356,P<0.05). The relative level of WT1gene≥1 in all of the 6 relepasing patients, which have a low remission rate when retreatment.Conclusions:Detection of the expression of WT1 gene and proteins could be used as a means to assess the clinical prognosis in the patients with AML. WT1 protein can serve as targets for AML immunotherapy.