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The Protective Effect Of HWTX-I On The Damage Of Free Radical In Rat Brain Mitochondria After Cerebral Ischemia-reperfusion

Posted on:2006-12-07Degree:MasterType:Thesis
Country:ChinaCandidate:H Y ZhouFull Text:PDF
GTID:2167360155456351Subject:Human Movement Science
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Competitive sport match is full of fierce antagonism and competitiveness. It is the fact that athletes will be often wounded or disabled caused by the sport damage in various kinds of exercise and compete. And cranium brain damage is comparatively common in the athletic injury, serious cranium brain damage will cause ischemia and lack of oxygen in central nervous system, causing neuron apoptosis and necrosis, which has a high rate of injury and mortality. This research attempts to probe the protective effect of HWTX-I on lipid peroxidation reaction which is intervened by oxygen free radical in central nervous system through the impact of cerebral ischemia on free radical of mitochondria in brain cortex of rat and on cellular shape of hippocampi CA1, in order to offer the experimental basis for the clinical practice and treatment of HWTX-I in brain damage.Methods 32 grown-up male SD rats, which is divided into sham-operation group (n=8), control group (n=8), higher dosage of HWTX-I (1μg/kg) group (n=8) and lower dosage of HWTX-I (0.5μg/kg) group (n=8) at random. We congeal electrically two-side vertebra arteries, insert anaesthesic tube into intrathecal space and separate two-side carotids on all of rats except the rats of sham-operation group. Among them, the rats of sham-operation group is sutured directly, and two-side carotids of the rest rats are closed for 7-15 minutes with undamaged blood vessel clip in order to make a model of global cerebral ischemia. Then inject 0.9% NaCl into the rats of control group immediately and inject 1μg/kg or 0.5μg/kg of HWTX-I into higher or lower dosage group respectively after unclamping blood vessel clip. After 24 hours, we execute all of the rats by decollating, distill mitochondria in the brain and determine the content of MDA and activation of SOD and GSH-Px; On the other hand, get the brain issue of rats again after the reperfusionoperation to prepare the samples under electron microscope, paraffin sectioning to observe the situation of apoptosis of brain cell under microscope and the sub-micro structural change of brain cell under the electron microscope. Results(1) The obvious pathological change takes place in the brain issue of control group under the microscope, the nucleus is concentrated and dyed deeply, the cellular interval is enlarged. The neuron is damaged in different dosage of HWTX-I groups after cerebral ischemia, but it is less serious than that of control group.(2) Under the electron microscope, observing that the chromatin is evenly distributed, the membrane of nucleus and mitochondria is completed and the mitochondria is clear to see in sham-operation group. But in control group, the membrane of nucleus is partially dissolved, the membrane of mitochondria is collapsed and the chromatin get together to the edge and which is partially collapsed. However, in HWTX-I groups, the shape of nucleus is regular, the membrane system is integrated, the chromatin is less together.(3) In the control group, the activation of SOD in brain mitochondria has been reduced by 44% and it has a significant difference (p<0.Ql), if compared with sham-operation group. In higher dosage group, it has been increased by 51% and the difference has significance (p<0.01) if compared with control group. But the difference between different dosage groups is not remarkable.(4) In the control group, the content of MDA in brain mitochondria has been obviously increased than that of sham-operation group, showing that it can cause lipid peroxidation damage after cerebral ischemia and reperfusion. But it has been reduced in brain mitochondria of rat after injecting different dosages of HWTX-I, which has been reduced by 60% in higher dosage group (lug/kg)...
Keywords/Search Tags:Sprague-Dawley rat, Huwentoxin-I, mitochondria, cerebral ischemia and reperfusion, free radical
PDF Full Text Request
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