Influencing Study Of Endurance Training On ATGL,HSL,CGI-58,Perilipin Gene Expression In Rat Gastrocnemius And Soleus

Recent study has discovered that Adipose Triglyceride Lipas(eATGL)is the key enzymes of fat hydrolysis. The hydrolysis of Adipose Triglyceride is a series of orderly controlled processes with different lipases involved in each process. Intramuscular Triglyceride (IMTG) can provide energy for the muscle contraction by hydrolysis and oxidation when in motion. Thus the regulation of ATGL expression is very much concerned. ATGL mRNA,HSL mRNA,CGI-58 mRNA and Perilipin mRNA expression of rat gastrocnemius and soleus are closely related to keeping homeostasis and eubolism of body energy. Meantime, exercise and training have a great influence on the changing of IMTG hydrolysis regulating gene expression in fast-twitch and slow-twitch muscles.Therefore, in this experiment, we choose male Sprague-Dawley rats as the subject of research, which were randomly assigned and divided into training groups of low-intensity, medium-intensity and high-intensity incremental endurance. Bedford's method was used to set exercise load with different exercise intensity grades on rats. The rats were put on the treadmill for different incremental treadmill endurance training; after 5 weeks endurance training, rats were killed. ATGL, HSL, CGI-58, Perilipin mRNA expression of rat gastrocnemius and soleus were evaluated by reverse transcription polymerase chain reaction (RT-PCR), the following is the main results:1. ATGLmRNA expressions both in rat gastrocnemius and soleus reached maximum value during the medium intensity incremental endurance training(P<0.05); there was no statistical discrepancy for ATGLmRNA expression between rat gastrocnemius and selous at the same endurance training(P >0.05). This indicates that the medium intensity incremental endurance training has the greatest impact on fast-twitch and slow-twitch muscles; and suggesting that ATGLmRNA expression of fast-twitch and slow-twitch muscles be consistent by reaction to incremental endurance training.2. During the three trainings, intensity incremental endurance training induced that there was some difference for HSLmRNA expression between rat gastrocnemius and selous.The expression of rat gastrocnemius and selous reached maximum value at the high-intensity incremental endurance training (P<0.05);but there was no difference between rat gastrocnemius and selous for HSLmRNA expression at the same endurance training(P >0.05). This indicates that HSL mRNA expression of muscle are related to different intensity exercise.3. In the experiment, with gastrocnemius and selous under the same training, CGI-58mRNA expression in gastrocnemius reached maximum value at high-intensity incremental endurance training, which was significantly higher than in soleus(P<0.05). CGI-58 mRNA expression of rat selous reached maximum value at the medium-intensity incremental endurance training,but there was no statistical discrepancy for each group. The fact illustrates that the phenomenon is related to the sensitivity of exercise intensity and the difference of CGI-58 mRNA expression between fast-twitch and slow-twitch muscles only appeared at the high-intensity incremental endurance training.4. In the study, the gastrocnemius and the soleus were based on fast-twitch slow-twitch muscle fiber ,when they were trained by the incremental endurance training methods, the relative expressions of the mRNA expression between rat gastrocnemius and soleus had no significant transformation. When the gastrocnemius and soleus were trained at the same endurance training, the differences of the Perilipin mRNA expression of two muscles were not significant, either (P>0.05). This indicates that the reaction of Perilipin mRNA expression of fast-twitch to incremental endurance training is consistent with slow-twitch muscles.