Re Amino Acid Heterocyclic Ternary Complexes Of Synthesis, Characterization And Related Biological Activity

Ten new solid ternary complexes of rare earth ions (RE= La³⁺,Ce³⁺,Pr³⁺,Nd³⁺,Sm³⁺,Eu³⁺,Tb³⁺,Dy³⁺,Er³⁺,Y³⁺) with L-Aspartic acid (L-Asp) and o-phenanthroline (Phen) were synthesized in ethyl aqueous solution. The compositions of the complexes were confirmed to be: RE(Asp)3PhenCl3·3H2O base on the XRD, Elemental analysis, Molar conductance, FT-IR,Raman, UV-VIS, 1HNMR etc. The stability of heat was proved by the data of TG-DTA. Four dinuclear complexes were synthesized (Eu0.8La0.2(Asp)3PhenCl3·3H2O, Eu0.8Y0.2(Asp)3PhenCl3·3H2O, Eu0.8Tb0.2 (Asp)3PhenCl3·3H2O, Eu0.8Dy0.2(Asp)3PhenCl3·3H2O). From the fluorescence spectra, we can conclude that La³⁺, Y³⁺, Tb³⁺, Dy³⁺ all could enhance the fluorescence of Eu³⁺ in the order of La³⁺>Y³⁺>Tb³⁺>Dy³⁺.The actions of the rare earth (La³⁺,Eu³⁺,Tb³⁺,Dy³⁺,Y³⁺) complexes and CTDNA with UV-Vis spectra were researched. When the concentrations of the complexes are increased, the largest absorption in A260 is gradually Red shift and also weak. This experiment indicates that the flat aromatic structure of o-Phenanthroline ligand of the complex may overlap with the stacking base pairs of DNA by intercalation. The antibacterial activities of the complexes were evaluated by the agar-diffusion method, Minimum Inhibitory Concentration and surge–flask method. The antibacterial activities testing showed that all these complexes exhibited excellent antibacterial ability against Escherichia coli, Staphylococcus aureus and Candida albicans with broad antimicrobial spectrums. Meanwhile, the complexes are durable quality. We use Invert Microscope (IM), Fluorescence microscope (FCM) and Methyl Thiazolyl Tetrazolium (MTT) colorimric to test the anti-tumour effect of the complexes with K562 tumor cell. The rearch shows that the complexes can inhibit K562 tumor cell's growth, generation and induce apoptosis. And there was significant difference compared with control group. Inhibition ratio accrescenced by dosage's increasing, and it had significant positive correlation with medication dosage (r=0.67). The median inhibitory dose (ID50) is 0.02 mg·ml-1 and when the time of action is 72h, the inhibition ratio is biggest.