Study On Hybridization Breeding Of Xinjiang Wild Argali

This research project was carried out to use the unique conditions of keeping Xinjiang wild argali in the Shihezi West Park. A preliminary study on the electro-ejaculation, necrozoospermia treatment, semen cryopreservation, estrus synchronization, superovulation technology, and blood biochemical indices of hybrid generation sheep were measured and analyzed, obtain certain results.1. Electro-ej aculation and necrozoospermia treatment of argaliElectric stimulation was used to collection semen of argali, has collection semen 59 times in total,53 times was successful, the success rate was 89.8%. Electric stimulation different output voltage can caused varying degrees of erectile response to argali, with the increasing number of semen collection, about the 8v output voltage stimulation can caused more easily penile erection and ejaculation response. Seven years old argali after treatment, sperm count increased significantly and activity was significantly enhanced, so that fertilization pregnant ewes. Three years old argali after the treatment, sperm count and activity have no significant change. Successfully collected the argali semen, sperm motility and density were better, indicating that stimulation has no effect on semen quality of argali, electro-ejaculation is suitable method for argali semen collection.2. Study on argali frozen semen technologyTo accurately evaluate the effect of argali frozen semen, we designed three dilution formulas, the four corners of domestic sheep and argali semen was diluted semen, frozen, preserved and thawing under the same conditions.3# diluent or Tris 3.63g, glucose 0.50g, fructose 1.99g, sodium citrate 0.63g, taurine 0.63g, penicillin 100,000 IU, streptomycin 100,000 IU, egg yolk 15ml, glycerol 12% frozen semen after thawing, sperm activity of argali and the four corners of domestic sheep sperm corners were 0.23±0.06 and 0.31±0.15, the effect was better. Argali frozen thawed semen viability was significantly lower than the four corners of sheep, in addition to the four corners of fresh and dynamic than sheep argali addition, it also breeds, physiological structure and environmental factors, yet to be studied further.3. Study on Estrus synchronization techniqueUsing CIDR, PG, PMSG, LHRH-A3 of reproductive hormones on argali, Bashibai sheep and the F1 generation of sheep and other breeds of sheep were synchronization of estrus. Estrus rate of argali was 75%, F1 generation of sheep was 68%, Bashibai sheep was 80%, 93,33% for other breeds of sheep. Estrus rate of argali, F1 generation of sheep was significantly lower than other breeds of sheep, the difference was very significant (P<0.01); Bashibai sheep was significant compare to F1 generation of sheep and other species sheep were significant differences (P<0.05); argali and F1 on behalf of the sheep was no significant difference, shows the genetic stability of argali, hybrids already has the physical characteristics of the argali.4. Study on superovulation techniqueUsing CIDR, PG, PMSG, FSH, LHRH-A3 of reproductive hormones on argali, F1 generation of sheep, domestic sheep was proceed superovulation. Ovulation among argali, F1 generation of sheep and domestic sheep were very significantly difference (P<0.01); the average ovulation of argali was very significantly difference compared to the F1 generation of sheep and domestic sheep (P<0.01), F1 generation of sheep and domestic sheep were significant difference (P<0.05); Successful rate of superovulation and the average number of argali ovulation were 75%(3/4) and 3.3/head, F1 generation of sheep was 100%(3/3) and 16/head, domestic sheep was 100%(3/3) and 20.7/head, obtain better superovulation response in generally.5. Measurement and analysis of blood physiological parametersThe RBC, Hb and PCV of argali were significantly higher than Bashibai sheep, F1 and F2, the difference was very significantly (P<0.01).PLT,MCH,MCHC of argali, Bashibai sheep and F1 were significantly lower than F2, the difference was very significantly (P<0.01),among the argali, Bashibai sheep and F1 were no significant difference (P>0.05).WBC between argali and Bashibai sheep was no significant (P>0.05), but were significantly higher than the F1 and F2, the difference was very significantly(P<0.01); there was no significant difference between F1 and F2sheep (P>0.05).LYM, MID and GRAN of argali was significantly higher than Bashibai sheep, F1 and F2, the difference was very significantly (P<0.01), while among Bashibai sheep, F1 and F2 was no significant difference (P>0.05).6. Measurement and analysis of blood biochemicalGLU of argali was higher than Bashibai sheep, the difference was significant (P<0.05); Bashibai sheep was significantly lower than F2, the difference was very significantly(P <0.01).TB and DB of argali were significantly lower than the Bashibai sheep, the difference was very significantly (P<0.01); Bashibai sheep was significantly higher than F2, the difference was very significantly (P<0.01); F1 was significantly higher than the F2, the difference was very significantly (P<0.01).ALT of argali was significantly higher than the Bashibai sheep, the difference was very significantly (P<0.01); Bashibai sheep was lower than F2, the difference was significant (P <0.05); F1 was lower than argali, the difference was significant (P<0.05).Content of various mineral elements, in addition to sodium content higher than normal reference values, the remaining elements of the content although the argali, Bashibai sheep, F1 and F2, although some differences exist, but were within the normal range of sheep, and the six elements changes of F1, F2 with the characteristics of argali, indicating that argali and their hybrids strong adaptability to the environment, future generations of sheep genetic stability.