Hubei The Ophiopogon Key Planting Technology Research

Objective: Studies on key planting technologies of Root of Liriope spicata (Thunb.) Lout. vat. prolifera Y. T. Ma were carried out to normalize the planting and direct the generalization of good species.Methods:The seedlings were identified into the following four categories: seedlings with coarse roots and newborn buds(1), seedlings with coarse roots, rootlets and newborn buds(2), seedlings with only rootlets(3), seedlings with rootlets together with long rhizome(4). Different seedlings are planted into quadrats respectively. Seedlings with rootlets, coarse roots and newborn buds were used in the next two experiments. To select a reasonable planting density, we designed four planting densities as follows, 10cm in plant spacing×15cm in row spacing(5), 15cm×20cm (6),20cm×20cm(7),22cm×22cm(8). In the experiment on scapes, we compared Root of Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma whose scapes were picked off in anthesis (9) with those weren't(10). All the above ten kinds of samples were planted into quadrats in Xiang Fan respectively, and every quadrat has three copies. Each quadrat had an area of 2.1m×5.6m, and the whole field was 30×(2.1m×5.6m) in area. All Liriope spieata (Thunb.) Lour. var. prolifera Y. T. Ma were under the identical field management including watering, fertilization and anti-pest measures. Ripe root tubers were collected and prepared on March 39, 2006.HPLC-ELSD was used to determine the content of Liriopesides B of the ten samples above. The chromatographic condition was as follows: Chromatographic column: Alltech C₁₈(4.6mm×250 mm, 5μm ); The temperature of the column: 40℃; Mobile phase: acetonitrile-methanol-water (65∶5∶35); Mobile phase rate: 1 ml/min. The parameters of detector ELSD were: Drift tube temperature: 40℃; Gas pressure: 3.5 bar. Phenol-sulphuric acid colorimetric method was use to determine the content of polycose. The content of the two kinds of active compounds were used in quality evaluation.Additionally, we determined the output of each quadrat and calculated output of each acreage according to the area and planting density.Data annlysis used SPSS 13.0. One-Way ANOVA method was used to compare the quality and output of different seedlings and planting densities. Independent-Sample T Test method was used to compare samples under different scape-treating measures.To decide the collection period, we collected the root tubers once every 20 days since their intumescence for 6 times. We determined the content of Liriopesides B, average output of single plant, yield of dried root tubers and research their diversities accompanied to the time.Results:Comparisons among seedlings: (â…°) the content of Liriopesides B: sample 1＞(s2, s3)＞s4. (â…±) the content ofpolycose: s2＞(s1, s3, s4). (â…²) output: indiscriminative。Comparisons among planting densities: (â…°) the content of Liriopesides B: (s5, s6)＞s7＞s8. (â…±) the content ofpolycose: (s5, s7, s8)＞s6. (â…²) output: s5＞s6＞s7＞s8.Contrasts between different scape-treating measures: (â…°) the content of Liriopesides B: s9＞s10. (â…±) the content ofpolycose: s9＞s10. (â…²) output: s9＞s10.Liriope spicata (Thunb.) Lour. var. prolifera Y. T. Ma showed great differences in different periods. The content of Liriopesides B increased obviously since Mar. 20 while the average output of single plant and the yield of dried root tubers touched their peak since Mar. 20 and Jan. 30 respectively. Considering the results as well as the farmers' benefits, the first quarter of April was regarded the best collection period.Conclusion:Fine seedlings: seedlings with coarse roots and newborn buds, seedlings with coarse roots, rootlets and newborn buds.Reasonable planting density: 10cm in plant spacing×15cm in row spacing.Better scape-treating measure: scapes picked off in anthesis.Best collection period: the first quarter of April.