In Vitro Culture Of Spores And Establishment Of Rapid Propagation System Of Adiantum Flabellulatum L.

In vitro culture of Adiantum flabellulatum L.was studied in this paper.Spores, prothallus and sporophyte were used as explants for the culture.The effects on spore germination of several factors including light,inorganic salt,sucrose and storage period were studied.The GGB(green globular bodies) of Adiantum flabellulatum was successfully induced in the different culture conditions,and the different media with different types of hormone concentrations and the mix of different tests.In vitro propagation system of Adiantum flabellulatum was established,which provided important data for the rapid propagation of other ferns.The main results were as follows:The Adiantum flabellulatum spores were filtrated after immersion in 75%ethanol for 15s and then in 0.1%HgCl₂ solution for 4 minutes.This was the optimal sterilization method for Adiantum flabellulatum spores.1/4 strength MS medium containing sucrose 15 g l^-1～45 g l^-1 was the appropriate medium for spores germination and prothalli development of Adiantum flabellulatum.A definite concentration of sucrose was of benefit to the development of meristem and the differentiation of sex organ.The suitable culture condition for spores germinating was normal illumination(illumination intensity 1000 lux～1500 lux) and dark condition made against the germination of spores.Storage at 4℃was of benefit to spores germination.The germination rate of spores stored at 4℃for 8 months was the highest,and after 8 months the germination rate rapidly declined.MS medium containing NAA 0.5 mg l^-1 was the appropriate medium for Adiantum flabellulatum prothallus proliferation.There was no remarkable difference in the number of sporophyte induced between incomplete differentiation culture and normal culture.MS medium containing BA 1.0 mg l^-1 was the most effective and economical medium for inducing GGB of Adiantum flabellulatum and the corresponding proliferation multiple was 6.1.Illumination had no effect on GGB inducement.The most suitable concentration of Zeatin for proliferation of sporophyte was 1.0 mg l^-1,the corresponding proliferation multiple was 3.6.MS medium containing BA 1.0 mg l^-1 and NAA mg l^-1 was the appropriate medium for GGB proliferation of Adiantum flabellulatum,the corresponding proliferation multiple was more than 40.ZT 0.1 mg l^-1 was the optimum cytokinin concentration for GGB differentiation.Acclimation in sand bottles before transplanting could remarkably increase the survival rate to 90%.