| Theory of Lung and Large Intestine organs in Chinese medicine is one of the relevant theory, which embodies the whole concept of thinking in Chinese medicine.This theory originated in the "Yellow Emperor", after the ancient physicians through continuous clinical practice, and gradually confirm and deepen the scientific nature of this theory. With the development of modern medicine, especially in immunology, cell biology, molecular biology and other disciplines, people take modern medical means to deepen and enrich the "Lung and Large Intestine" theory of knowledge, some success has been achieved both in theory and clinical.1 ObjectiveThe topic of "Lung and Large Intestine" theory as the theoretical basis of the relevant literature, from the perspective of mucosal immunity and receptor between the lung and large intestine relationship in order to reveal the "Lung and Large Intestine" theory of modern science content, enrich theoretical and experimental studies of lung and colon-related in traditional Chinese medicine theories organs, provide further reference and support, as well as clinical diagnosis and treatment of intestinal related lung disease to provide a theoretical reference.2 Methods2.1 TheoryWe use literature data and the combination of logical analysis to explore the role of mucosal immunity and receptor expression in lung and large intestine role relationship, indicate that it is significant to take relevant research from the perspective of mucosal immune and receptor, which will reveal the basic lung and Large Intestine Important way content.it has further enriched the theory of Chinese medicine organs, to provide reference for other organs theoretical research related.2.2 Experimental study2.2.1 Animals Healthy SD rats, female,50, weight 200±20g.Randomized into 5 groups of 10 each, respectively, for high oxygen group, hypoxic group, starvation group, constipation group and the normal control group.high oxygen group, kept in a closed box of oxygen, oxygen concentration 40%, free food and water intake, feeding for 5 days; hypoxic group, kept in a closed box of oxygen, oxygen concentration 10%, free food and water intake, feeding for 5 days; starvation, kept at room temperature,1/2 the average food intake, divided morning and evening feeding, free water intake, feeding 7 days; constipation group, kept at room temperature,1/2 the average amount of water, divided morning and evening giving, free food intake, feeding 7 days; normal control group, kept at room temperature, free food and water intake, feeding 7 days.These groups are natural light, normal atmospheric pressure, humidity 50%-70%, temperature 22' C-25℃. High-oxygen group, hypoxic group than in the other three groups two days later began formal experiment, five groups at the same time drawn. Abdominal aort ic blood were killed, lungs, jejunum, ileum, colon, rectum, kidney (the first five drawn); lavagefluid, intestinal mucus (the second batch of five drawn)2.2.2 DetectionThese indicators are used enzyme linked immunosorbent assay(ELISA).2.2.3 Statistical analysis of experimental resultsAll data are mean±standard deviation with (X±S).SAS8.2 for analysis. Compare between each group or in the same group with single factor analysis of variance (One-Way ANOVA) tests, to P<0.05 as significant difference.3 Results3.1 Theoretical resultsChinese medicine in the "lungs" of modern medicine, including respiratory system and its functions, Chinese medicine in the "large intestine" of modern medicine, including the colon, rectum and part of the small intestine and their functions. Table between the lung and large intestine is an important part of the relationship between the organs one table, they contact each other through the meridians in the physiology and pathology.Lung and large intestine links on the interaction of a variety of mechanisms and material manifestation. Mucosal immunity and receptors is an important material basis for lung and large intestine associating. It is feasible and important to research the relationship between lung and large intestine contents of modern biological mechanism through mucosal immunity and receptor.3.2 Experimental results3.2.1 Mucosal immunity resultsThe results show that high oxygen group, hypoxic group, starvation group, constipation group sIgA in BALF were higher than the normal control group. the high oxygen group, hypoxic group, starvation sIgA was signifi-cantly higher than the normal control group(P<0.05); high-oxygen group, hypoxic group content of intestinal mucus sIgA was significantly lower than the control group (P<0.05), and starvation group, constipation group content of intestinal mucus sIgA was significantly higher than the control group (P<0.05)High-oxygen group content of intestinal mucus and lavage fluid for sIgA IL-1βIL-2 IL-6 TNF-αwere significantly lower than the control group, corresponding to the first content related indicators (P<0.05)Hypoxia group IL-1βin BALF were significantly higher than the normal control group (P<0.05), IL-1βin the intestinal mucus content was lower than the normal control group, no significant difference (P>0.05); Intestinal mucus and lavage fluid for IL-2, IL-6 levels were significantly lower than the control group(P<0.05); in the lavage fluid and mucosal mucus content of TNF-a were significantly higher than the control group (P<0.05)Starvation group in the lavage fluid and mucosal mucus content of the index compared with the control group, TNF-αin intestinal mucus were higher than the control group (P<0.05), the remaining lavage fluid and intestinal mucus IL-1β, IL-2, IL-6, TNF-αlevels were lower than the normal control group.Intestinal mucus IL-2 content compared with the control group was no significant difference (P>0.05), other than the normal control group were significantly different (P<0.05)Constipation group the lavage fluid and intestinal mucus content of each indicator compared with the control group, IL-1βin BALF mucosal mucus content and TNF-αlevels were higher than the corresponding control group (P>0.05), the remaining lavage fluid and intestinal mucus of each index were significantlylower than the control group (P<0.05)3.2.2 Receptor resultsThe results showed that all groups with the lung content of IL-1βreceptor compared to the jejunum, ileum, colon, rectum, IL-1βreceptor was significantly higher than the content of the lungs(P<0.05), IL-1βreceptor content in the kidney is lower than the content of lungs, no significant difference (P>0.05); jejunum, ileum, colon, rectum, IL-6 receptor was significantly higher than the content of the lungs (P<0.05); IL-6 receptors in the kidney were significantly lower than the content of the lungs (P<0.05)Jejunum, ileum, colon, rectum, TNF-αreceptor was significantly higher than the content of the lungs (P<0.05); kidney TNF-αreceptor levels were lower than the content of the lungs, no significant difference (P>0.05)4 Conclusionlung and intestinal sIgA is an important immune material basis of functional linkages, which is a positive antibodies of contacting the benign lung and intestinal.when rats sustained in the environment of high oxygen, hypoxia, starvation constipation, immune cytokine in lung and intestinalmucosal was declining in the overall consistency, to some extent, which revealed the relationship of lungs and the large intestine.The IL-1βmay be a sensitive mucosal immune cytokine in the lungs, so the higher levels in the lungs; TNF-αin the mucosal immune response to less severe conditions may play a protective role in lung and intestine, therefore the higher levels in the lung and intestinal tract.Lung, intestine, kidney-related cell factor receptor content in order as follows:gut>lung>kidney. Jejunum, ileum, colon, rectum, the overall content of the cytokine receptor related keep consistent, which to some extent, reveal that the large intestine in Chinese medicine includes colon, rectum, the part of jejunum and ileum in modern medicine. The gut associated high levels of cytokine receptors, which maybe related to intestinal cells re-absorption of the free-type relevant cytokine receptor in the body. Lung-related cytokine receptor levels were lower than the gut maybe related to mechanism that lung adjusts water-superssion and transfports cloud fluid to the bladder, since the cloud liquid may contain some free-type cytokine receptors.Kidney-related cytokine receptors was the lowest, which maybe related the detoxification function of the kidney itself. the kidney maybe eliminate some of the relevant cytokine receptors from the organ itself.Mucosal immunity and receptors is important mechanism associated with lung and large intestine. However, as relations between lung and large intestine is the result of joint action with a variety of substances and mechanisms, only through the point of mucosal immunity and receptor research, can' twe fully reveal the relationship of modern biology mechanism between them, to be coming from which should be carried out comprehensive research under a broader Level. |
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