| Cerebrovascular diseases (CVD) is one of the three main reasons of deathfor the high morbidity,mortality and mutilation rate .The intracerebral hemorrhage(ICH) is one of the most critical diseases.After intracerebral hemorrhage, neuron bring about pathophysiology changes for cerebral edema,brain hematoma and toxic action of metabolite. Investigation in this field can provide direct andcertain evidence. In Chinese medical theory, the essential mechanism of stroke is the origin deficiency and excessin superficiality. The location is in brain. Jiunaoning injection (JNN) had functions of removing bloodstasis and detoxifying, clearing away heat , dispering phlegm ,calming the wind and resuscitation. Thebrain protective effects and mechanism of Jiunaoning injection were studied by animal and cellculture experiments in this thesis that guided by Chinese medical theory.1 Effects of Jiunaoning injection on experimental cerebral hematoma in rabbits The model of experimental cerebral hematoma was produced by injecting autogenous clot in thebrain of rabbits. Male rabbits were divided into the six groups of normal, model, JNN (large, middle,small dosage) and Oingkailing injection (QKL). JNN and QKL were administrated by ear veininjection, and all of the experimental indicators were detected on the 3rd, 7th and 15th day.1.1 The effect of JNN on nerves function disability and cerebral edema of ICH in experimentalrabbits Results showed that brain index, content of water in the groups of JNN and QKL decreasedsignificantly, especially in the group of JNN of middle dosage. The symptoms of nerves function inthe three dosage groups of JNN improved remarkably. These shows JNN injection can accelerateabsorption of cerebral edema and resist progress of cerebral edema .1.2 The effect of JNN on brain tissue morphological changes and nerve cell apoptosis of ICHexperimental rabbits Thebraintissuesliceswerestained by HE, Nissl and Mallory stainfor the histochemistric studies.The model group was found that the neuronal morphologic changes in ICH included numerousnecrosis of nerve cells and infiltration of inflammatory cells in hematoma region. Neuron lost partly,remained cells swollen, acidophilia change of cytoplasm, pyknosis of nucleus and reduce of Nisslbody in remained cells and glialcell reaction n in this area. These changes in the three dosage groupsof JNN and QKL were smaller than that in the group of model on the 3rd,7th and 15th day. Theseresults indicated that JNN could improve brain tissue morphological changes of experimental ICHrabbits. The terminal-deoxynucleotibyl transferase mediated nick end labeling(TUNEL) wereemployed to detect the nerve cells apoptosis in the brain paraffin sections. Results showed that thepositive cells of TUNEL stain increased notably in model group. Apoptosis could be found in all partsof cerebral edema zone. Compared with group normal, the positive cells of model group increased-4- 救脑宁注射液对家兔脑血肿及离体培养神经细胞损伤的作用机制研究significantly, while compared with group model, it decreased in group JNN (middle dosage) onthe15th day. It suggested that JNN could restrain nerve cells apoptosis.2 protectiveeffects of JNNon oxygen/glucose-deprived and reperfusion injury neuron In vitro, primary cultured neuron was replaced culture medium with hypoxia and hypoglycose,incubated in an atmosphere of 93%N2, 7%CO2 for 4 hours at 37℃ namely "ischemia", returned tonormalmediumfor4hoursnamely"reperfusion", This is model imitatingischemiareperfusion(I/R) .2.1 Effects of JNNon neuronal metabolism viability and LDH leak rate in ischemia-reperfusion injuryinduced neuron Various concentrations of Jiunaoning injection (0.5-5μl/ml) were used to observe their effects onoxygen/glucose-deprived and reperfusion injury induced ratcorticalneuronsinvarioustimepoints(2,4,6h), the neuronal metobolism viability in primary cultures from rat cerebral cortex was assessed u... |
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