Green Qin Liquid Damage Repair Role Of Hyperuricemia In The Rat Model Of Autoimmune Joint Pathology

Objective:Hyperuricemia is a level of uric acid in the blood that is abnormally high.In humans,the upper end of the normal range is 360μmol/L(6mg/dL) for women and 420μmol/L(7mg/dL) for men.Causes of hyperuricemia can be classified into three types:increased production of uric acid,decreased excretion of uric acid,and mixed type.Gout is a crystal deposition disease hallmarked by elevated levels of uric acid in the bloodstream.In this condition,crystals of monosodium urate(MSU) or uric acid are deposited on the articular cartilage of joints,tendons and surrounding tissues.The principal medications used to treat hyperuricemia act by decreasing the blood concentration of uric acid.These are xanthine oxidase inhibitors,which reduce the production of uric acid;and uricosurics,which reduce the reabsorption of uric acid excreted by the kidneys. For acute gouty arthritis treatments,the first line of treatment should be pain relief.Once the diagnosis has been confirmed,the drug options are of nonsteroidal anti-inflammatory drugs (NSAIDs),colchicine and oral glucocorticoids,or intra-articular glucocorticoids administered via a joint injection.Colchicine remains a second line drug for those unable to tolerate NSAIDs,it impairs the motility of granulocytes and can prevent the inflammatory phenomena that initiate an attack.However,its side effect profile has resulted in its role being relegated.Qing Qin Ye is based on Professor Meng's Traditional Chinese Medicine(TCM) prescription,which has obtained effective results from treating hyperuricemia and acute gouty arthritis.The following experiment will observe the inflammatory response on rats model with acute gouty arthritis from treatment of Qing Qin Ye.Methods:Laboratory animals were divided into normal group,model group,colchicine group,Qing Qin Ye high-dosage group,Qing Qin Ye mid-dosage group and Qing Qin Ye low-dosage group. Adenine and ethambutol were given to all groups except normal group.Colchicine 0.09 mg/kg·d were administered to the rats of colchicine group.Groups of Qing Qin Ye high-dosage group were administered Qing Qin Ye 5g/kg·d,mid-dosage group were administered 2.5g/kg·d and low-dosage group were administered 1.25g/kg·d.All methods were administered by gavage.After 1 week,sodium urate was injected into all rats' ankle cavity except normal group.72 hours later,determine the level of Interleukin-8(IL-8) and Tumor Necrosis Factor(TNF) by ELIS A method.Then used 10%formalin to fix ankle joints, decalcify,paraffin embed,and HE dyeing.Pathological section with articular cavity and surrounding tissues were made to observe the inflammatory response.Results: Between all tested groups,level of IL-8 and TNF-αhad no significant differenciation (P＞0.05).However,the contents of IL-8 in Qing Qin Ye low-dosage group were lower than model group,colchicine group,Qing Qin Ye high dosage group and Qing Qin Ye middle dosage group.Contents of TNF-αin all Qing Qin Ye groups were lower than model group and colchicine group,especially with Qing Qin Ye mid-dosage group.Pathological section with articular cavity and surrounding tissues were better in colchicine group and all Qing Qin Ye groups.Conclusions:1.Qing Qin Ye can restrain IL-8 and TNF-αactivity;decrease inflammatory response from acute gouty arthritis.2.Qing Qin Ye can remedy fiberized in surrounding tissues;can remedy swelling,hyperemia and pain.3.Qing Qin Ye is a better curative than colchicine.