Toxic Elimination Liver Granule Liver Function In Rats With Hepatic Encephalopathy, Ammonia And Endotoxin Level

Objective:In order to observe the effect of Duxiao Ganqing Keli which fluences liver function consisting of ALT,AST,TBIL,ALB,BA,EEG,LPS,IL—6,TNF-a in the animal model of experimental hepatic encephalopathy in rats and effect of Duxiao Ganqing Keli to gastrointestinal function,furtherly to discuss the therapeutic effect and mechanism in HE.Methods:1.Dividatur the rats into 6 groups randamly:blank array,model array,Lactitol array,the diminutive,midst,large dose of Duxiao Ganqing Keli.2.Methods To induce the hepatic encephalopathy the rats were given intraperitoneal injection with.TAA.3.After modeling success,the drugs Duxiao Ganqing Keli.were given to the latter three groups and Lactitol.we given to positive control group until 2 weeks.The normal group was not any treatment and the model group through stomach feeding.by physiological saline.4.After 2 weeks,Execute the rats in full after determining the electroencephalogram, abdominal aorta blood was drawn,we test liver function and the serum Ammonia concentration.Observe the contents of LPSin hypothalamus of the hepatic encephalopathy rats model with enzyme linked immunosorbent assay(Elisa) We observe the effects of LPS,by Duxiao Ganqing Keli treatment of HE in rats.Results:1.The effect on the weight,behavioristicsa of HE rats model after Duxiao Ganqing Keli's treatment:Therapeutic groups rats weight gain and nerves reflex enhance,the midst,large dose of Duxiao Ganqing Keli were statistically significant,the lactitol array,the diminutive one were meanningful than than the model array's(P＜0.01).the large one was with significant difference in hepatic encephalopathy stages change,and there isn't significant difference in the diminutive one with Lactitol array.2.The effect on the liver function,LPS,and the serum Ammonia of HE rats model after Duxiao Ganqing Keli's treatment:All the indexes decreased notably after treatment to the therapeutic groups than the model array's(P＜0.05),and liver function,BA,LPS decreased notably in the midst,large dose of Duxiao Ganqing Keli,there were significant differences than the model array's(P＜0.01).It showed certain improvement after Duxiao Ganqing Keli's treatment. Conclusion:1.We can produce the hepatic encephalopathy model of rats successfully by the rats were given intraperitoneal injection with TAA.2.The consequence to the research manifest that:The consequence to the research manifest that:Duxiao Ganqing Keli can improve hepatic function,BA,LPS of the HE rats in a certain extent,the midst,large dose of Duxiao Ganqing Keli is efficient.On the whole, it is effective than the positive control group.3.Duxiao Ganqing Keli is a sort of active drug used to prevention and cure the hepatic encephalopathy,and is worthy of further study and development.