| Objective:Removing liver clearing Pill on serum tumor necrosis factor alpha in rats with liver cirrhosis were observed by virus(TNF- alpha), interleukin-6(IL-6) and the pathological changes of the liver, further proof of duxiao Ganqing role Pill on treatment of liver cirrhosis and its mechanism.Methods:1 The experimental rats were randomly divided into 6 groups: blank group, model group, lactulose group, duxiao Ganqing pill high, medium and low dose group.2 Model: except the blank group rats in other groups were given subcutaneous injection of 50% carbon tetrachloride solvent thigh, each 0.5 ml- 100g-1, 2- WK-1, and according to the change of body weight adjusted dose, a total of 12 wk, preparation of liver cirrhosis model.3 Intragastric administration: In addition to the blank group and model group were given distilled water, the rats were treated with corresponding drugs orally. Blank group: at the same time reared under the same conditions, the daily given distilled water intragastric administration of 2ml/100g; Model group: at the same time reared under the same conditions, the daily given distilled water intragastric administration of 2ml/100g; High dose group(223.4mg / kg): once a day by intragastric administration, continuous administration of 12 weeks; The middle dose group(111.7mg / kg): once a day by intragastric administration, continuous administration of 12 weeks; Low dose group(58.9mg / kg): once a day by intragastric administration, continuous administration of 12 weeks;Lactulose group(concentration 100g/L, 1ml/100 g by gavage): once a day by intragastric administration, continuous administration of 12 weeks.4 In the continuous intragastric administration for 12 weeks, all rats were killed, blood, were measured by using enzyme linked immunosorbent assay(enzyme linked immunosorbent assay, ELISA) detection of serum levels of TNF- alpha and IL-6 of the rats, and observe the effects of duxiao Ganqing Pill on liver cirrhosis in rats serum TNF- alpha and IL-6 levels.5 By means of SEM duxiao Ganqing Pill on liver cirrhosis in rats pathological morphological changes and their impacts on the.Results:1 Effects of duxiao Ganqing Pill on the serum levels of IL-6 alpha, TNF- alpha in rats with liver cirrhosis: duxiao Ganqing pill high, medium dose group compared with the model group rats serum TNF-alpha, IL-6 content decreased significantly(P<0.01), drug consumption decreased liver clearing pill low dose group rats serum TNF- alpha, the content of IL-6(alpha P<0.05), compared with the control group had no significant difference in drug. Description of duxiao Ganqing Pill on liver cirrhosis in rats serum TNF- alpha, IL-6 content has a certain improving effect.2 Effects of duxiao Ganqing Pill on pathological changes of rat liver with cirrhosis: lactulose group, high dose group, middle dose group and low dose group of liver cirrhosis in rats and the protective effect of reducing.Conclusion:1 The thigh subcutaneous injection of 50% carbon tetrachloride solvent, each 0.5 ml-100g-1, 2- WK-1, and according to the change of body weight adjusted dose, 12 wk, can be successfully prepared animal models of hepatic cirrhosis model.2 Determination of the indicators in this study showed that: duxiao Ganqing pill can to a certain extent, reduce the level of TNF- alpha, IL-6, improve the morphology of rats with liver cirrhosis liver pathology, duxiao Ganqing pill high dose group, obvious curative effect, and the overall effect is better than lactulose group.3 Effects of duxiao Ganqing Pill on pathological changes of rat liver with cirrhosis: lactulose group, high dose group, middle dose group and low dose group of liver cirrhosis in rats and the protective effect of reducing. |
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