| ObjectiveThe calyx seu fructus physalis (jingdenglong) is the traditional chinese herbal medicine, and be documented in all the past Chinese Pharmacopoeia. There is not sutible method to control its quality. So in present thesis we study calyx seu fructus physalis the deals with the isolation, content determinations, chromatographic, Growth factors and so on. To Study the chemical constituents in-deep, to provide quality contral mark, to establish the quality standards, to determine the optimum harvest period and storage conditions.In addition,to expand the resources for meditionl of physalis.,study the chemical constituents of stem and leaves.Material and method:Material:The calyx seu caulis and leaf of phyhalis alkekengi L.var.franchetii (Mast.) Makino. of solanaceae.Method: 1. The chemical constituents of calyx seu and caulis and leaf of phyhalis alkekengi L were isolated by silica gel column chromatography and sephadex LH-20 chromatography technique. On the bases of physic-chemical property and spectroscoie and chemical evidence their structures were identified.2. The chromatographic fingerprint is established by HPLC. The materials are contributed different habitat and harvested in different time and different part of plant were detected. In the mean time, HPLC is carried out to deteminated the content of above materials.Result and Conclusion:1 Big amount of Physalin A, Physalin O, Physalin L, Physalin B, Physalin L, luteolin-7-0-0 -β-D-glucopyranoside were isolated form the caly sue physalis that can provide chemical mark for he quality control. In addition ten of eleven compounds also isolated from the clay sue physalis were identified on the basis of physics and chemistry constant, spectral analysis as well as chemistry method. Including 25,27-dehydro-physalin L were new natural products, and 22 silane acid and Cuneatasid E were isolated from calyx physalis for the first time.2 Twelve compound were isolated from the stems and leaves of phyhalis alkekengi L.seven of them were identified as Tricosanoic Acid, ombuine, Physalin B, physaflavonol, Physalin A, Physalin O, Physalin L, Physalin D.Comparing with clay sue,the main chemical composition is similar,but little difference.3 The fingerprint of the drug of 20 regions of product is established and four physalins were set as the quality standards. The study of fingerprint and content determination indicate that the quantity quality is different significantly among the different habitats and different storage time. We suggest that the valid storage time of clay sue physalis is about 2 years of herbs.4 The storage conditions of physalis alkekengi L was studied for the first time. Result showed that the conditions have significant influence with the contents of physalins. The physalins is sensitive to the temperature, ultraviolet ray and mould.All of them can make the quality of physalis alkekengi L degenerate. So the storage method of clay sue physalis should avoid light and mouldproof at ordinary temperature.In addition, the clay sue physalis should be separated from fruits and placed in the shade and ventilation to dry after harvesting time.5 The chromatographic fingerprint of different harvesting time of sue and stems and leaves of physalis alkekengi L were established and the variations of main effective ingredients were determined. According to the change of contents of the effective ingredients with harvesting time, the optimal harvesting time is late August to early September. |
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